Figure 5From: Identification of a novel biomarker candidate, a 4.8-kDa peptide fragment from a neurosecretory protein VGF precursor, by proteomic analysis of cerebrospinal fluid from children with acute encephalopathy using SELDI-TOF-MS Purification of the 4.8-kDa biomarker candidate using HPLC. The 4.8-kDa protein eluted from the Q Sepharose Fast Flow anion exchange column in the 100 and 200 mM NaCl fractions. The 200 mM NaCl fraction eluted from the Q Sepharose Fast Flow anion exchange column was further purified by reverse phase HPLC.Back to article page