Photomicrographs of frozen skin sections (50 μm) from a Fabry patient. Samples immunoreacted with PGP 9.5 and were processed for fluorescence microscopy with Cy3 labelled secondary antibodies. Note the lack of intraepidermal nerve fibers and persistence of fibers pertaining to the subepidermal nerve plexus (arrows) in the sample from the lower leg skin of a Fabry patient (upper figure). Note the dense innervation of the epidermis (arrows) in the sample from the back of the Fabry patient, taken at the dermatome Th 12 (lower figure). Bar = 50 μm.