Figure 5

Optic nerve sections were stained for myelin by eriochrome (blue stain) and AQP4 expression 62 days after injection of human IgG or saline. The mice that received injections of NMO-IgG show areas of pale blue demyelination (arrows in A). The control-IgG group had some demyelination in the optic nerves (arrows in B) and vehicle controls had only few visible demyelinating lesions (panel C). Similar to the spinal cord, AQP4 staining (anti-AQP4, Santa Cruz, white) was increased in all groups in areas of demyelination (sections D-F). Scale bars represent 100 μm.