Transverse cryosections of VL muscle biopsies from an OPMD patient over a 6 year interval. A) Biopsy taken at the time of first presentation in the clinic. B) Biopsy of the same muscle as in (A) taken 6 years later. Sections were stained with haematoxylin-eosin. Muscle fibrosis is evidenced by fat infiltration (F), vacuole with basophilic rim (arrowhead) and thickening of the ECM (arrows). Scale bar equals 100 μm.