Figure 6
From: Analysis of IFT74as a candidate gene for chromosome 9p-linked ALS-FTD
(A) Human brain soluble extracts (40 ug per lane) were separated on 4–20% SDS-PAGE gels and blotted using a goat polyclonal antibody to the C-terminus of IFT74 (Imgenex, anti-CMG1) at a final antibody concentration of 0.5 mg mL-1. Two major bands at ~90 kDa and ~70 kDa were identified which were blocked by pre-absorption of the antibody with the immunizing peptide (data not shown), indicating specificity. (B) A. Immunofluorescent staining of primary rat cortical neurons with the same antibody showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B. Secondary antibody alone gave no signal using consistent gain and offset settings.