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Table 2 Protein changes unique to RIA, UIA, or IA

From: Identification of the soluble form of tyrosine kinase receptor Axl as a potential biomarker for intracranial aneurysm rupture

Protein name iTRAQ ratios a Nb
DC RIA UIA
Proteins unique to RIA     
Immune/inflammatory response     
  Complement C1s subcomponent precursor 1.03 ± 0.10 1.28 ± 0.16 0.96 ± 0.11 4
  Pigment epithelium-derived factor precursor 1.29 ± 0.16 0.76 ± 0.05 1.22 ± 0.25 4
Cell Adhesion     
  Alcadein alpha-1 1.22 ± 0.17 0.81 ± 0.03 1.32 ± 0.11 2
  Calsyntenin-1 precursor 0.78 ± 0.16 1.20 ± 0.12 0.82 ± 0.08 3
  Contactin 2 precursor 0.93 ± 0.14 1.20 ± 0.08 0.95 ± 0.21 5
  Nectin-like protein 2 1.09 ± 0.15 0.61 ± 0.13 1.62 ± 0.23 3
  Neural cell adhesion molecule 1, 140 kDa isoform precursor 0.88 ± 0.12 0.44 ± 0.14 0.97 ± 0.21 3
Extracellular Matrix     
  Secretogranin III precursor 0.83 ± 0.05 0.63 ± 0.04 1.00 ± 0.16 3
  Splice isoform 1 of fibrinogen gamma chain precursor 0.96 ± 0.15 1.52 ± 0.19 1.18 ± 0.12 3
  Splice isoform 1 of fibulin-1 precursor 1.03 ± 0.12 0.64 ± 0.11 1.09 ± 0.15 3
Protein synthesis or protein degradation     
  Hypothetical protein FLJ23322 0.64 ± 0.09 1.43 ± 0.14 0.46 ± 0.05 2
  Hypothetical protein FLJ23757 1.07 ± 0.08 3.79 ± 0.53 0.99 ± 0.10 2
  PREDICTED: KIAA1509 0.64 ± 0.18 1.43 ± 0.11 0.46 ± 0.27 2
  Splice isoform 1 of ecto-ADP-ribosyltransferase 3 precursor 1.20 ± 0.16 3.05 ± 0.31 1.15 ± 0.23 2
  TAR RNA loop binding protein 0.71 ± 0.25 1.50 ± 0.16 1.14 ± 0.08 3
Transport     
  Afamin precursor 1.18 ± 0.08 1.39 ± 0.05 1.19 ± 0.12 4
  Ceruloplasmin precursor 1.28 ± 0.14 0.76 ± 0.19 0.94 ± 0.19 10
  Neuronal pentraxin I precursor 1.07 ± 0.10 0.67 ± 0.04 0.88 ± 0.03 2
  Serotransferrin precursor 0.98 ± 0.47 0.76 ± 0.50 0.96 ± 0.35 59
Apoptosis or anti-apoptosis     
AXL receptor tyrosine kinase, isoform 1 1.12 ± 0.11 1.68 ± 0.07 0.87 ± 0.16 2
  Splice isoform 1 of amine oxidase flavin containing domain protein 2 0.88 ± 0.13 0.38 ± 0.14 1.12 ± 0.08 2
  Beta-2-glycoprotein I precursor 0.85 ± 0.14 1.48 ± 0.20 1.09 ± 0.09 4
  Ephrin A1 isoform b precursor 0.75 ± 0.06 1.23 ± 0.03 1.18 ± 0.02 2
  Tyrosine phosphatase zeta polypeptide 2 HTPZP2 1.32 ± 0.21 0.43 ± 0.12 1.20 ± 0.19 2
Cell proliferation/differentiation/migration  
  Splice isoform 1 of erythrocyte membrane protein band 4.2 0.93 ± 0.09 3.86 ± 0.49 1.53 ± 0.11 2
Signal transduction     
  XPR1 protein 0.88 ± 0.18 0.38 ± 0.07 1.12 ± 0.15 2
Metabolism     
  Guanylate binding protein 4 1.26 ± 0.13 0.68 ± 0.10 1.07 ± 0.11 2
  Splice isoform 2 of adenosine kinase 0.88 ± 0.08 0.38 ± 0.15 1.12 ± 0.11 2
  Splice isoform 2 of phospholipid transfer protein precursor 0.71 ± 0.23 1.45 ± 0.07 0.86 ± 0.16 2
Cell structure/cytoskeleton     
  Nebulin 1.11 ± 0.21 1.74 ± 0.30 1.18 ± 0.09 3
  PREDICTED: dynein, cytoplasmic, heavy polypeptide 2 0.88 ± 0.09 0.38 ± 0.12 1.12 ± 0.16 2
  Splice isoform 3 o myosin Va 0.68 ± 0.29 1.55 ± 0.15 1.14 ± 0.08 3
  Titin 1.08 ± 0.14 0.64 ± 0.07 1.07 ± 0.13 2
Unknown     
  17-beta hydroxysteroid dehydrogenase 0.80 ± 0.21 1.75 ± 0.23 1.12 ± 0.16 2
  Chromosome 1 open reading frame 27 0.88 ± 0.17 0.38 ± 0.12 1.12 ± 0.16 2
  JRK protein 1.05 ± 0.05 1.77 ± 0.17 0.69 ± 0.06 2
  PREDICTED: KIAA1522 protein 0.60 ± 0.16 1.40 ± 0.20 0.84 ± 0.12 2
  PREDICTED: similar to RIKEN cDNA 1700022C21 0.80 ± 0.21 1.75 ± 0.16 1.12 ± 0.10 2
  Taste receptor type 2 member 7 0.88 ± 0.12 0.38 ± 0.06 1.12 ± 0.04 2
  Zinc finger MYND domain containing protein 19 0.97 ± 0.03 1.31 ± 0.12 0.86 ± 0.08 2
Proteins unique to UIA     
Immune/inflammatory response     
  Complement C5 precursor 1.34 ± 0.15 1.09 ± 0.07 0.68 ± 0.05 2
Cell Adhesion     
  Splice isoform 1 of neogenin precursor 1.05 ± 0.10 1.10 ± 0.14 0.71 ± 0.12 3
Protein synthesis or protein degradation     
  Hypothetical protein FLJ34458 1.13 ± 0.10 0.94 ± 0.12 0.63 ± 0.03 2
  SERPIND1 protein 1.01 ± 0.15 1.00 ± 0.08 1.21 ± 0.06 4
Transport     
  Transthyretin precursor 0.95 ± 0.15 0.88 ± 0.07 0.69 ± 0.11 5
Apoptosis or anti-apoptosis     
  Baculoviral IAP repeat-containing protein 4 1.20 ± 0.11 1.00 ± 0.06 0.47 ± 0.08 2
  Nectin-like protein 2 1.09 ± 0.15 0.61 ± 0.13 1.62 ± 0.23 3
  Nucleolysin TIAR 0.93 ± 0.07 0.88 ± 0.12 0.68 ± 0.06 3
  Splice isoform 2 of signal transducer and activator of transcription 1-alpha/beta 1.13 ± 0.06 0.94 ± 0.03 0.63 ± 0.10 2
Cell proliferation/differentiation/migration     
  Condensin subunit 2 0.85 ± 0.10 0.87 ± 0.09 0.55 ± 0.08 3
Signal transduction     
  Grb10 interacting GYF protein 1 1.13 ± 0.05 0.94 ± 0.14 0.63 ± 0.02 2
Metabolism     
  Vitamin D-binding protein precursor 1.14 ± 0.10 1.06 ± 0.18 1.29 ± 0.09 6
Unknown     
  JRK protein 1.05 ± 0.05 1.77 ± 0.17 0.69 ± 0.06 2
Proteins changed both in RIA and UIA     
Immune/inflammatory response     
  Complement C3 precursor 0.91 ± 0.40 1.28 ± 0.42 1.25 ± 0.33 16
  Splice isoform 2 of interleukin-17E precursor 1.21 ± 0.09 0.62 ± 0.13 0.67 ± 0.12 2
Cell Adhesion     
  Neurocan core protein precursor 0.91 ± 0.15 0.44 ± 0.10 0.54 ± 0.12 3
  Splice isoform 2 of fibrinogen alpha/alpha-E chain precursor 1.14 ± 0.16 0.56 ± 0.12 0.78 ± 0.13 4
Extracellular Matrix     
  Extracellular matrix protein 1 1.16 ± 0.12 0.55 ± 0.15 0.69 ± 0.13 3
  Metalloproteinase inhibitor 1 precursor 1.07 ± 0.11 1.25 ± 0.08 1.31 ± 0.12 4
Protein synthesis or protein degradation     
  Acheron, isoform 1 0.97 ± 0.03 1.31 ± 0.08 1.64 ± 0.16 2
Transport     
  Apolipoprotein D precursor 1.19 ± 0.08 1.43 ± 0.12 1.47 ± 0.11 3
  Serum albumin precursor 1.08 ± 0.17 1.39 ± 0.11 1.59 ± 0.12 4
Cell proliferation/differentiation/migration     
  Septin 10 isoform 2 1.08 ± 0.02 1.28 ± 0.05 1.46 ± 0.08 2
Signal transduction     
  Adrenomedullin 2 precursor 1.08 ± 0.04 1.30 ± 0.07 1.28 ± 0.04 2
  CD59 glycoprotein precursor 0.90 ± 0.07 0.49 ± 0.03 0.64 ± 0.06 2
  Neuronal pentraxin receptor isoform 1 1.00 ± 0.02 0.74 ± 0.05 0.50 ± 0.11 3
Metabolism     
  Lumican precursor 0.99 ± 0.13 1.57 ± 0.04 1.36 ± 0.13 3
  N-acetylgalactosamine-4-O-sulfotransferase 0.88 ± 0.05 1.21 ± 0.08 1.28 ± 0.07 2
Cell structure/cytoskeleton     
  Keratin 1 1.49 ± 0.17 0.66 ± 0.07 0.82 ± 0.07 3
  PREDICTED: similar to keratin, type I cytoskeletal 18 (cytokeratin 18) (K18) (CK 18) 1.73 ± 0.28 0.69 ± 0.06 0.76 ± 0.08 2
Unknown     
  DJ570F3.6 0.69 ± 0.23 2.36 ± 0.36 2.14 ± 0.24 2
  MUF1 protein 1.09 ± 0.08 0.72 ± 0.10 0.72 ± 0.11 3
PREDICTED: hypothetical protein XP_291007 0.97 ± 0.06 0.59 ± 0.04 0.60 ± 0.04 2
  1. aiTRAQ ratios shown as mean ± S.D. obtained by averaging the iTRAQ ratios of individual peptides. The differences between groups were analyzed by one–way ANOVA followed by Newman-Keuls multiple comparison test. bNumbers of identified peptides used for calculating the iTRAQ ratios. The iTRAQ ratio in bold showed significant differences compared to the other group(s) (p < 0.05).