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Table 2 Primary antibodies used in the present study

From: Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal β-amyloid deposition in the human brain

Antibody

Source

Product #

Dilution

Mouse anti-Aβ1-42, 12 F4

Signet

39240

(1:2000)

Mouse anti-Aβ1-16, 6E10

Signet

39320

(1:4000)

Rabbit anti-β-actin

Sigma-Aldrich

A2066

(1:5000)

Mouse anti-amyloid precursor protein (APP), 22C11

Millipore

MAB348

(1:1000)

Rabbit anti-amyloid precursor protein (APP), C-terminal

Edward Koo

CT15

(1:2000)

Rabbit anti-amyloid precursor protein (APP), C-terminal

Serotec

AHP538

(1:2000)

Mouse anti-alpha-smooth muscle actin (αSMA)

abcam

ab7817

(1:2000)

Rabbit anti-BACE1α (a.a. residues 46-163)

Huaibin Cai

anti-BACE1α

(1:2000)

Mouse anti-CD31

Dako

M0823

(1:1000)

Mouse anti-collagen, type IV

Sigma-Aldrich

C1926

(1:1000)

Mouse anti-fibronectin

Sigma-Aldrich

F7387

(1:2000)

Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH)

Millipore

MAB374

(1:10000)

Mouse anti-glial fibrillary acidic protein (GFAP)

Millipore

MAB3402

(1:2000)

Mouse anti-microtubule associated protein-2 (MAP2)

Sigma-Aldrich

M9942

(1:1000)

Rabbit anti-presenilin-1 N-terminal fragment (PS1-NTF)

Samuel Gandy

Ab14

(1:500)

Rabbit anti-phospho-Tau (p-Ser199/Ser202) (p-Tau)

Sigma-Aldrich

T6819

(1:3000)

Mouse anti-synaptophysin (SYN)

Millipore

MAB329

(1:4000)

Rabbit anti-β-tubulin-III

Sigma-Aldrich

T2200

(1:10000)