Fig. 2

The immunoreactivity of patient’s serum to CASPR2 and LGI1 proteins. EU90 cells were transfected with cDNAs encoding CASPR2, LGI1 and other four neuropil proteins associated with autoimmune encephalitis (EUROIMMUN, FA 112d-1005-1, Germany), incubated with this patient’s serum and detected by IIFT. This patient’s serum collected at different time showed different immunoreactivity to CASPR2 (a, d, g) and LGI1 (b, e, h), and areas with no specific fluorescene to other proteins on the same slide tested at the same time would be regard as control (c, f, i). The patient’s serum collected on November 26, 2014 showed strong binding to the surface of cells expressing CASPR2 proteins (a) (50x) and moderate binding to the ones expressing LGI1 proteins (b)(50x). The patient’s serum collected on December 26, 2014 showed moderate binding to the surface of cells expressing CASPR2 proteins (d) (50x) and no binding to the ones expressing LGI1 proteins (e) (50x). The patient’s serum collected on January 12, 2015 showed weak binding to the surface of cells expressing CASPR2 proteins (g) (100x) and no binding to the ones expressing LGI1 proteins (h) (100x)