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Table 4 Blood sampling procedure

From: The Cognition and Affect after Stroke - a Prospective Evaluation of Risks (CASPER) study: rationale and design

Tubes Blood markers Procedure
EDTA tube (4 × 6.0 mL) Myeloperoxidase, neopterin, soluble intercellular adhesion molecule, soluble vascular cell adhesion molecule-1, soluble E selectin, soluble P selectin, CD40 ligand, interleukin-6, tumor necrosis factor alpha, Homocysteine Transported on ice to the Biobank Maastricht UMC+ where the blood is preprocessed before storing. EDTA plasma is centrifuged and stored at −80 °C until analysis.
Natriumfluoride/EDTA tube (1x 4.0 mL) Coagulation tube (1 × 5.0 mL) Glucose, total cholesterol high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, triglyceride, folate, vitamine B12, high sensitivity C-reactive protein Direct analyses of these markers are performed at the laboratory on the day of sample collection after centrifuging.
Buffycoats from the 4 EDTA tubes for DNA extraction Apoliprotein E, clusterin, phosphatidylinositol binding clathrin assembly protein, encoding complement [3b/4b] receptor 1, angiotensin-converting enzyme,methylenetetrahydrofolate reductase Buffycoats are taken from four EDTA tubes and separately stored at the Biobank until analysis.
Epigenetics 1 PAXgene tube (2.5 mL) RNA Blood is collected in PAXgene tubes to ensure long-term stability of ribonucleic acid and the tubes are stored at the Biobank. RNA extraction and subsequent analysis of expression of inflammatory and stroke-related genes at the mRNA level will be done.
  1. Abbreviations: mL milliliter, EDTA ethylenediaminetetraacetic acid, DNA deoxyribonucleic acid, RNA ribonucleic acid, mRNA messenger ribonucleic acid
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