Fig. 2
From: New multiplex real-time PCR approach to detect gene mutations for spinal muscular atrophy
Detection SMN, NAIP and GTF2H2 genes by multiplex PCR and DNA Sanger sequencing. a The DNA sequence result of wild-type at c.840 of SMN (exon7); (b) The DNA sequence result of homozygous mutation of C > T at c.840 of SMN (exon7); (c) The multiplex PCR image for NAIP and GTF2H2 gene deletions. M, Marker; a, NAIP exon4; b, GTF2H2 exon10; c, NAIP exon5; (d): The single PCR image for wild type and NAIP or GTF2H2 gene deletions. M, Marker; 1, 3, 5, samples of wild type of NAIP and GTF2H2; 2, patients with NAIP exon5 deletion; 4, patients with NAIP exon4 deletion; 6, patients with GTF2H2 exon10 deletion; (e) The DNA sequence map of NAIP exon4; (f) The DNA sequence map of NAIP exon5; (g) The DNA sequence map of GTF2H2 exon10