Fig. 4
Measurement of absolute cerebral blood flow (CBF) in ischemic striatum. a CBF was measured in ischemic striatum (ipsi) and the corresponding area of the contralateral hemisphere (contra) at four weeks after 30 min MCAo using the 14C-iodoantipyrine tissue equilibration technique. The experiments depicted here as well as in Figs. 5 and 6a were conducted in the same animals. Briefly, of 10 VEC+/+ and 16 VEC+/- mice entered into the experiment, 2 VEC+/+ and 5 VEC+/- mice died after MCAo. One further VEC+/+ mouse died during preparation for blood flow experiments. Moreover, due to technical issues with the femoral artery catheter, one VEC+/+ and one VEC+/- animal each could not be used for measurement of CBF. The analysis presented here is therefore based on 6 VEC+/+ and 10 VEC+/- mice. Finally, the ipsilateral side of the brain of one further VEC+/- mouse sustained substantial damage during cryostat sectioning, so the ipsilateral hemisphere of this animal is also not included here. Two-way ANOVA revealed an overall effect of factor ‘genotype’ on CBF. Newman-Keul’s post hoc test, *p < 0.05 between genotypes within the ischemic striatum. Representative pseudocolored autoradiographic images of coronal brain sections are shown in (b). Scale bar in (b) 1 mm