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Fig. 2 | BMC Neurology

Fig. 2

From: Long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibits proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway

Fig. 2

LncRNA PTCSC3 overexpression inhibits the proliferation of U87 and U251. a qRT-PCR analysis of lncRNA PTCSC3 expression levels in U87 and U251 infected with Lenti-Vector or Lenti-PTCSC3. CCK-8 assays were performed to determine U87 (b) and U251 (c) cell proliferation after 12 h, 24 h, 48 h and 72 h infected with Lenti-Vector or Lenti-PTCSC3. d Representative flow cytometry images of the cell cycle in U87 and U251 cells infected with Lenti-Vector or Lenti-PTCSC3. The cell cycle results quantified in of U87 (e) and U251 (f) cells are presented as the percentage of total cells. g Colony formation assay was performed to determine the proliferation of U87 and U251 cells infected with Lenti-Vector or Lenti-PTCSC3. The colonies were captured and counted. Colony formation assay results of U87 (h) and U251 (i) cells are presented as histograms. Data are expressed as the means ± S.D of three independent experiments. “*” indicates P < 0.05, “**” indicates P < 0.01

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BMC Neurology

ISSN: 1471-2377

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