Fig. 4

Notch1 was a direct target of miR-139-5p. (a) Diagram of the seed sequence of miR-139-5p matched the 3′-UTR of Notch1. (b and c) qRT-PCR and Western blotting for Notch1 expression after transfection with miR-139-5p or miR-Scr. (*P < 0.05. ***P < 0.001). (d) Schematic diagram of the design of wild or mutant Notch1 3′-UTR containing reporter constructs. (e and f) Luciferase reporter assays in LN229 and U87 glioma cells after co-transfection with wild-type or mutant 3′-UTR Notch1 and miRNAs. 3′-UTR-NC, Negative Control of Notch1 3′-UTR. miRNA-NC, Negative Control of miR-139-5p. The data represent the fold change in the expression (means+SE) of 3 replicates (*P < 0.05)