Table 1 Primary antibodies used in our two autopsy cases for diagnosis
Antibodies | Dilution | Company | Findings in Case 1 and Case 2 |
|---|---|---|---|
GFAP | 1:300 | DAKO, Glostrup, Denmark |  + in the reactive astrocytes |
NeuN | 1: 500 | Millipore, Temecula, USA |  + in the neurons |
Neurofilament (NF) | 1: 2000 | DAKO, Glostrup, Denmark |  + in the axons and axonal spheroids |
Phosphorylated NF | 1: 10,000 | Millipore, Temecula, USA |  + in the axons and axonal spheroids |
Synaptophysin | 1: 100 | Novocastra, Newcastle, UK |  + in the gray matter |
TMEM | 1: 500 | ABCAM, Bristol, UK |  + in the intrinsic microglia |
CD163 | 1: 200 | ABCAM, Bristol, UK |  + in the pigmented microglia |
CD68 | 1: 2000 | DAKO, Glostrup, Denmark |  + in the exogenous macrophages |
CD3 | 1: 100 | DAKO, Glostrup, Denmark | Negative in the entire brain |
CD20 | 1: 100 | DAKO, Glostrup, Denmark | Negative in the entire brain |
α-synuclein | 1: 200 | ABCAM, Bristol, UK | Negative in the entire brain |
β-amyloid | 1: 500 | Covance, Dallas, USA | Negative in the entire brain |
3 repeat (3R) tau | 1: 100 | Millipore, Ontario, Canada | Negative in the entire brain |
4 repeat (4R) tau | 1: 1000 | Millipore, Ontario, Canada | Negative in the entire brain |
p-Tau (AT8) | 1: 100 | ThermoFisher, Waltham, USA, | Negative in the entire brain |
p-TDP43 | 1: 1,000 | Cosmobio, Tokyo, Japan | Negative in the entire brain |