Fig. 4

DNMT1 siRNA prevented LPS-induced M1 polarization and accelerated M2 polarization in HAPI cells. A RT-qPCR determined the expression of DNMT1 mRNA in HAPI cells; B Western blot determined the protein level of DNMT1; C-D Flow cytometry detected the percentages of M1 microglia phenotype (CD16) and M2 microglia phenotype (CD206); E Ratio of M1/M2 microglia; F RT-qPCR measured the relative mRNA levels of iNOS and Arg-1; G ELISA determined the levels of TNF-α, IL-1β, IL-6, and IL-10. Cell experiment was conducted 3 times; data were shown as mean ± SD. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001