Fig. 5

DNMT1 siRNA promoted microglia M2 polarization by blocking the PI3K/Akt pathway. A-C Protein levels of PI3K, p-PI3K, Akt, and p-Akt measured by Western blot. D-G M1 microglia phenotype (CD16) and M2 microglia phenotype (CD206) detected using flow cytometry; H Ratio of M1/M2 microglia; I The relative mRNA levels of iNOS and Arg-1 measured by RT-qPCR; J Levels of TNF-α, IL-1β, IL-6, and IL-10 determined by ELISA. Cell experiment was conducted 3 times; data were exhibited as mean ± SD. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001