Fig. 2

Histopathological results. Histopathological findings of the (A–B) brain tumor and (D–F) lymph nodes. Hematoxylin and eosin (HE) staining shows highly atypical tumor cells with areas of necrosis and microvascular proliferation (A: ×100, B: ×400). GFAP immunostaining is slightly positive in larger tumor cells, but negative in most cells (C: ×200). HE staining reveals cells with distinct nucleoli, nodding nuclei, and abundant eosinophilic endoplasmic reticulum, similar to brain tumor cells (D: ×400). The immunostaining results are (E) p53 positive, (F) GFAP-negative, (G) LCA-negative, and (H) CD20 negative (×200)