Potential risk factors for diabetic neuropathy: a case control study
BMC Neurology volume 5, Article number: 24 (2005)
Diabetes mellitus type II afflicts at least 2 million people in Iran. Neuropathy is one of the most common complications of diabetes and lowers the patient's quality of life. Since neuropathy often leads to ulceration and amputation, we have tried to elucidate the factors that can affect its progression.
In this case-control study, 110 diabetic patients were selected from the Shariati Hospital diabetes clinic. Michigan Neuropathic Diabetic Scoring (MNDS) was used to differentiate cases from controls. The diagnosis of neuropathy was confirmed by nerve conduction studies (nerve conduction velocity and electromyography). The multiple factors compared between the two groups included consumption of angiotensin converting enzyme inhibitors (ACEI), blood pressure, serum lipid level, sex, smoking, method of diabetes control and its quality.
Statistically significant relationships were found between neuropathy and age, gender, quality of diabetes control and duration of disease (P values in the order: 0.04, 0.04, < 0.001 and 0.005). No correlation was found with any atherosclerosis risk factor (high BP, hyperlipidemia, cigarette smoking).
In this study, hyperglycemia was the only modifiable risk factor for diabetic neuropathy. Glycemic control reduces the incidence of neuropathy, slows its progression and improves the diabetic patient's quality of life. More attention must be paid to elderly male diabetic patients with poor diabetes control with regard to regular foot examinations and more practical education.
Diabetes mellitus (DM) is one of the most widespread chronic diseases in the world. Nearly 7.5% of Iranian people are affected by DM type II . DM has two types of complications: microvascular and macrovascular. One of the most frequently-occurring microvascular complications is diabetic neuropathy (DN), of which the most common type is distal symmetrical neuropathy or polyneuropathy. This results in significant disability and morbidity [2, 3]. Complications of DN include severe pain, loss of ambulation and increased risk of foot ulceration and amputation.
Incidences of polyneuropathy have been reported in 10–50% of patients with diabetes . At the time of diagnosis, neuropathy is present in 10% of diabetic patients and overall in 50% of patients with a 25-year history of the disease [4, 5]. Life-time risk of foot amputation is 15% in patients with diabetic polyneuropathy . Polyneuropathy is the first step in the generation of diabetic foot ulcer. It produces an anesthetic foot defective in proprioception and therefore exposed to inappropriate loading. Foot ulcers develop in risk areas that are exact pressure points .
Different hypotheses have been proposed to explain the various modes of progression of DN. It has been suggested that consumption of oral hypoglycemic agents such as glyburide  and angiotensin converting enzyme inhibitors (ACEI) inhibit the progression of neuropathy irrespective of blood glucose level [8–10]. Atherosclerosis risk factors are thought to promote DN . The induction of mononeuropathy is closely associated with high blood pressure (BP), hyperlipidemia and cigarette smoking . Since neuropathy can lead to ulceration and amputation, we have tried to assess the relationships between these risk factors and sensory/motor polyneuropathy. Early diagnosis and treatment of DN is important for preventing secondary complications and improving quality of life.
One hundred and ten diabetic patients participated in this case-control study (55 patients in each group). Controls and cases were chosen from the Shariati Hospital diabetes outpatient clinic by simple randomized sampling. A control subject was a diabetic patient with no evidence of DN, and a case was a diabetic patient with neuropathy. The patients' ages ranged between 20 and 80 years. Exclusion criteria were creatinine >2 mg/dl, specific neurological disease (M.S, stroke, etc.), other causes of neuropathy (B12 deficiency, alcoholism, etc.), loss of dorsalis pedis pulses and less than 5 years duration of disease. Informed consent was completed by all participants before they were enrolled in the study. The study design was approved by the research ethics committee of the Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences.
In order to differentiate between cases and controls, Michigan Neuropathic Diabetic Scoring (MNDS) was used . This system gives a score in the range 0–8, based on evaluation of 4 different factors in the each leg. These factors are: appearance of foot (dry skin, callus, deformities, fissure, and infection), presence of ulcer, Achilles tendon reflex and vibration perception in the great toe (measured with a 128 Hz tuning fork). Each component may be given a score of 0.5 or 1 on the basis of the relevant signs. This scoring system has sensitivity and specificity of nearly 95% . A neuropathic foot usually scores 3 or higher, a normal foot 2.5 or lower. Cases with a diagnosis of neuropathy according to the Michigan scoring system were confirmed by nerve conduction studies (EMG-NCV). For this purpose, nerve conduction velocity, amplitude, duration and latency were assessed in 5 sensory/motor nerves (Median, Ulnar, Tibial, Proneal and Sural) on the non-dominant side of the body. At least one abnormal test in more than one of these nerves was considered indicative of neuropathy .
Detailed information on each patient's age, sex, type and duration of diabetes mellitus, mode of treatment (insulin, oral hypoglycemic agents or both), degree of blood glucose control (bad, fair, good), presence of hypertension, hyperlipidemia (serum total cholesterol level), ACEI consumption and smoking was recorded and compared between cases and controls.
The quality of diabetes control was classified according to the average glycosylated hemoglobin (HbA1C) over the previous year. Average HbA1C ≤7.5 was considered good quality control; average 7.6< HbA1C ≤9 was considered fair control, and average HbA1C ≥9.1 poor control. All necessary data were retrieved from the patient records in the diabetes outpatient clinic of Shariati Hospital. Patients with BP≥140/90 were considered hypertensive. Patients with total cholesterol ≥250 were considered hyperlipidemic. HbA1C was measured by HPLC. The total cholesterol level was measured by calorimetry (Pars Azmoon kit), and fasting blood sugar (FBS) by the glucose oxidase method (Pars Azmoon kit).
SPSS 10 software was used for data entry and analysis. Since multiple factors were analyzed, multivariate analysis was used. Logistic regression was the appropriate mode for analyzing the multiple risk factors in cases and controls
Of the 110 patients, 78% (79) were female and 22% (31) were male. The mean age was 55.1 ± 13.2 (20–80 years). All but one of the patients had type II DM. Mean fasting blood glucose and average duration of disease in the study population were 140.5 ± 8 mg/dl and 12.9 ± 7 years, respectively. Table 1 shows age, duration of disease, mean FBS, mean 2 hour post-parendial blood glucose (BS2hpp), HbA1C and total cholesterol in cases and controls. Table 2 shows the frequency of potential risk factors for polyneuropathy in the study population. No significant relationships were found between distal symmetric sensory/motor polyneuropathy and cigarette smoking, ACEI consumption, BP or cholesterol level.
Multivariate analysis revealed statistically significant relationships between DN and age, gender, degree of diabetes control and duration of disease (P values: 0.04, 0.04, < 0.001 and 0.005, respectively). Neuropathy was more frequent in men than women (odds ratio male/female 2.9, figure 1). The multivariate analysis results are shown in Table 3.
Poor diabetes control increases the likelihood of neuropathy 0.3 times (odds ratio good control/bad control 0.3 and fair/bad 0.2). Figure 2 shows the relationship between quality of diabetes control (good, fair and bad) and MNDS score. Each additional year of disease increases the likelihood of neuropathy 1.1-fold.
Diabetic polyneuropathy is a common complication of DM with high morbidity and impairment of quality of life. Tesfye et al.  studied 3,250 diabetic patients and reported an overall prevalence of peripheral neuropathy in 28% of them. The condition was significantly associated with age, duration of disease, height, diastolic blood pressure, smoking status, low HDL cholesterol level, high triglyceride level and HbA1C.
The Ashok study  showed significant relationships only with age and duration of disease. No other association was detected. Other studies have shown associations of neuropathy with age [14–19], duration of disease [14–20], metabolic control [15, 18–21], height [15, 22, 23], cigarette smoking [15, 19, 24], retinopathy [15, 21] and reduced HDL level . The results of the present study confirm previous reports regarding the association of neuropathy with male gender, age, glycemic control (HbA1C) and duration of disease. Our data are also concordant with the DCCT (Diabetes Control and Complications Trial)  and UKPDS (United Kingdom Prospective Diabetes Study) results , which used EMG-NCV to identify neuropathic patients. Our finding that male gender is associated with neuropathy is consistent with the DCCT report . Therefore, it can be concluded that MNDS criteria can be used with high confidence as an outpatient screening method. In our study, no statistically significant relationship was found between peripheral neuropathy and ACEI or consumption of oral hypoglycemic agents. Polyneuropathy was not significantly related to BP, smoking or hyperlipidemia. Most of our patients were nonsmokers, so it was impossible to examine the possible association between smoking and neuropathy critically.
Further studies using a randomized clinical trial are needed to evaluate the effects of ACEI and oral hypoglycemic agents on neuropathy.
Since hyperglycemia is a modifiable risk factor for diabetic neuropathy, intensive glycemic control is the most effective established therapy for reducing the incidence or slowing the progression of neuropathy and improving quality of life in diabetic patients. According to the results of the present study, better care should be given to elderly male diabetic patients with poor diabetic control in terms of regular foot examinations and more practical education.
Larijani B, Zahedi F: Epidemiology of diabetes mellitus in Iran. Iranian Journal of Diabetes and Lipid Disorders. 2002, 1: 1-8.
Braunwald E, Fauci AS, Kasper DL, editors: Harrison Principles of Internal Medicine. 2001, New York, McGraw- Hill, 15
Dyck PJ, Thomas PK: Diabetic Neuropathy. 1999, Philadelphia, WB. Saunders, 2
Dyck PJ, Kratz KM, Karnes JL, Litchy WJ, Klein R, Pach JM, Wilson DM, O'Brien PC, Melton LJ, Service FJ: The prevalence by staged severity of various types of diabetic neuropathy, retinopathy, and nephropathy in a population-based cohort: the Rochester Diabetic Neuropathy Study. Neurology. 1993, 43: 817-824.
Feldman EL, Russell JW, Sullivan KA, Golovoy D: New insights into the pathogenesis of diabetic neuropathy. Curr Opin Neurol. 1999, 12: 553-563. 10.1097/00019052-199910000-00009.
Kahn RC, Weir CG, ed: Diabetes Mellitus. 1994, Pennsylvania, Lea & Febiger inc, 13
Quasthoff S: The role of axonal ion conductances in diabetic neuropathy: a review. Muscle Nerve. 1998, 21: 1246-1255. 10.1002/(SICI)1097-4598(199810)21:10<1246::AID-MUS2>3.0.CO;2-B.
Martinez-Blasco A, Bosch-Morell F, Trenor C, Romero FJ: Experimental diabetic neuropathy: role of oxidative stress and mechanisms involved. Biofactors. 1998, 8: 41-43.
Qiang X, Satoh J, Sagara M, Fukuzawa M, Masuda T, Miyaguchi S, Takahashi K, Toyota T: Gliclazide inhibits diabetic neuropathy irrespective of blood glucose levels in treptozotocin-induced diabetic rats. Metabolism. 1998, 47: 977-981. 10.1016/S0026-0495(98)90354-7.
Cameron NE, Cotter MA, Horrobin DH, Tritschler HJ: Effects of alpha-lipoic acid on neurovascular function in diabetic rats: interaction with essential fatty acids. Diabetologia. 1998, 41: 390-399. 10.1007/s001250050921.
Feldman EL, Stevens MJ, Thomas PK, Brown MB, Canal N, Greene DA: A practical two-step quantitative clinical and electrophysiological assessment for the diagnosis and staging of diabetic neuropathy. Diabetes Care. 1994, 17: 1281-1289.
Lunetta M, Le Moli R, Grasso G, Sangiorgio L: A simplified diagnostic test for ambulatory screening of peripheral diabetic neuropathy. Diabetes Res Clin Pract. 1998, 39: 165-172. 10.1016/S0168-8227(98)00005-9.
Tesfaye S, Stevens LK, Stephenson JM, Fuller JH, Plater M, Ionescu-Tirgoviste C, Nuber A, Pozza G, Ward JD: Prevalence of diabetic peripheral neuropathy and its relation to glycemic control and potential risk factors. The Euro Diab IDDM complications study. Diabetologia. 1996, 39: 1377-1384. 10.1007/s001250050586.
Ashok S, Ramu M, Deepa R, Mohan V: Prevalence of neuropathy in type 2 diabetic patients attending a diabetes centre in South India. J Assoc Physicians India. 2002, 50: 546-550.
Maser RE, Steenkiste AR, Dorman JS, Nielsen VK, Bass EB, Manjoo Q, Drash AL, Becker DJ, Kuller LH, Greene DA, et al: Epidemiological correlates of diabetic neuropathy. Report from Pittsburgh Epidemiology of Diabetes Complications Study. Diabetes. 1989, 38: 1456-1461.
Boulton AJM, Knight G, Drury J, Ward JD: The prevalence of symptomatic diabetic neuropathy in an insulin- treated population. Diabetes Care. 1985, 8: 125-128.
Knuiman MW, Welborn TA, McCann VJ, Stanton KG, Constable IJ: Prevalence of diabetic complications in relation to risk factors. Diabetes. 1986, 35: 1332-1339.
Franklin GM, Kahn LB, Baxter J, Marshall JA, Hamman RF: Sensory neuropathy in non-insulin-dependent diabetes mellitus. The San Luis Valley Diabetes Study. Am J Epidemiol. 1990, 131: 633-643.
Barbosa AP, Medina JL, Ramos EP, Barros HP: Prevalence and risk factors of clinical diabetic polyneuropathy in a Portuguese primary health care population. Diabetes Metab. 2001, 27: 496-502.
Manuel Malacara J, Eugenia Davalos L, Cervantes F, Castillo J, Velasco E: Risk factors of the complications of diabetes mellitus. Rev Invest Clin. 1991, 43: 3-9.
Pirart J: Diabetes mellitus and its degenerative complications: a prospective study of 4400 patients observed between 1947 and 1973. Diabetes Care. 1978, 1: 168-188. 252-263
Hyllienmark L, Brismar T, Ludvigsson : Subclinical nerve dysfunction in children and adolescents with IDDM. Diabetologia. 1995, 38: 685-692.
Sosenko JM, Gadia MT, Fournier AM, O'Connell MT, Aguiar MC, Skyler JS: Body stature as a risk factor for diabetic sensory neuropathy. Am J Med. 1986, 80: 1031-1034. 10.1016/0002-9343(86)90661-3.
Eliasson B: Cigarette smoking and diabetes. Prog Cardiovasc Dis. 2003, 45: 405-413.
The DCCT Research Group: Factors in the development of diabetic neuropathy in feasibility phase of Diabetes Control and Complications Trial (DCCT). Diabetes. 1988, 37: 476-481.
Nasr CE, Hoogwerf BJ, Faiman C, Reddy SS: United Kingdom Prospective Diabetes Study (UKPDS). Effects of glucose and blood pressure control on complications of type 2 diabetes mellitus. Cleve Clin J Med. 1999, 66: 247-253.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2377/5/24/prepub
The authors are grateful to the Endocrinology and Metabolism Research Center (EMRC) for financial support of the EMG-NCV procedure.
The author(s) declare that they have no competing interests.
FB and FB: drafted the manuscript and coordinated the study
BL, MP, JL: conceived of the study and participated in the design of the study
MN: performed statistical analysis
All authors read and approved the final manuscript
Fargol Booya, Fatemeh Bandarian, Mohammad Pajouhi, Mahdi Nooraei and Jamshid Lotfi contributed equally to this work.
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Booya, F., Bandarian, F., Larijani, B. et al. Potential risk factors for diabetic neuropathy: a case control study. BMC Neurol 5, 24 (2005). https://doi.org/10.1186/1471-2377-5-24