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Increased plasma levels of epithelial neutrophil-activating peptide 78/CXCL5 during the remission of Neuromyelitis optica
© The Author(s). 2016
Received: 17 September 2015
Accepted: 23 June 2016
Published: 11 July 2016
In neuromyelitis optica (NMO), one of the underlying pathogenic mechanisms is the formation of antigen-antibody complexes which can trigger an inflammatory response by inducing the infiltration of neutrophils in lesions. Epithelial neutrophil-activating peptide 78 (ENA 78), known as Chemokine (C-X-C motif) ligand 5 (CXCL5), belongs to the ELR-CXCL family. It recruits and activates neutrophils. The aim of this study was to evaluate ENA 78, IL-1β and TNF-α plasma levels in multiple sclerosis (MS) and neuromyelitis optica (NMO) patients.
ENA 78, IL-1β and TNF-α plasma levels were detected in 20 healthy controls (HC), 25 MS and 25 NMO patients using MILLIPLEX® map Human High Sensitivity Cytokine/Chemokine Panels.
Plasma levels of ENA 78 were significantly higher in NMO patients than in HC (P < 0.001) and MS patients (P < 0.05). The NMO patients showed higher plasma levels of IL-1β compared with HC (P < 0.01). Further, increased plasma levels of TNF-α were found in the MS (P < 0.05) and NMO patients (P < 0.001). In addition, NMO patients had higher Expanded Disability Status Scale (EDSS) scores compared with MS patients (P < 0.05). EDSS scores were correlated with plasma levels of ENA 78 in NMO patients (P < 0.05). There were no significant correlations between EDSS scores and plasma levels of ENA 78 in MS patients (P > 0.05).
The overproduction of pro-inflammatory cytokines such as IL-1β and TNF-α during the remission of NMO activates ENA 78, which in turn leads to neutrophil infiltration in lesions. ENA 78 plasma levels were correlated with EDSS scores in NMO patients. Elevated secretion of ENA 78 may be a critical step in neutrophil recruitment during the remission of NMO.
KeywordsNeuromyelitis optica Epithelial neutrophil-activating peptide 78 Interleukin 1β Tumor necrosis factor α
Neuromyelitis optica (NMO, Devic’s syndrome) and multiple sclerosis (MS) are autoimmune and degenerative diseases characterized by demyelination of central nervous system (CNS), potentially leading to paralysis and other clinical symptoms [1–4]. NMO and MS are two of the most common diseases causing neurological disability in young adults [1, 5, 6]. Accumulating evidence has shown that NMO pathogenesis differs from MS, including aquaporin 4 (AQP4)-IgG increase and infiltration of granulocytes and macrophages [7, 8].
A significant feature distinguishing NMO from MS is the relatively higher number of neutrophils, eosinophils, macrophages and fewer T cells in the lesions [2, 8, 9]. Abnormal neutrophil aggregation in the lesions and increased AQP4-IgG are the notable features distinguishing NMO from MS [8, 10]. Neutrophil protease inhibition reduces AQP4-IgG damage in the mouse brain, which suggests that neutrophils play an important role in NMO pathology . Studies also suggest a tight regulation of neutrophils and immune cell recruitment in NMO . The innate immune response is orchestrated by inflammatory cells as a cascade of events, and each stage is associated with inflammatory cell recruitment and infiltration. Neutrophil infiltration is triggered by epithelial neutrophil-activating peptide 78 (ENA 78), which plays a role in tissue repair, metabolism, microbial killing, and angiogenesis [11, 12]. ENA 78 is a member of CXC chemokines, which enhance leukocyte recruitment and activation in autoimmune disorders and inflammatory diseases [13–15]. Its aberrant expression has been detected in rheumatoid arthritis, psoriasis, autism, bacterial meningitis, etc. [16–20]. ENA 78 is categorized into sub-classes based on the sequence and function, and characterized by the ELR (glutamic acid-leucine-arginine) motif preceding the N-terminal Cys and activating C-X-C chemokine receptor type (CXCR) 2 selectively . ENA 78 as a potent ELR+ CXC chemokine attracts and activates polymorphonuclear leukocytes (PMNLs) which are higher in patients with infections, inasmuch as the PMNLs are among the first cells to exist the peripheral blood and migrate to the inflammatory site [16, 22, 23].
This study was the first to evaluate plasma levels of ENA 78 and its relation to Expanded Disability Status Scale (EDSS) scores in NMO patients.
Written informed consent was obtained from all participants. The study protocol was approved by the local ethics committee (IRB of Beijing Tiantan Hospital Affiliated to Capital Medical University, No. KY2015-003-02). MS and NMO patients were recruited from Beijing Tiantan Hospital, Capital Medical University. This study was conducted between May and July 2015 on 20 healthy controls (HC), 25 patients with MS and 25 patients with NMO. Plasma samples were obtained from MS patients, NMO patients and 20 HC recruited from the general population without immune diseases. Infections were ruled out by full blood count in all subjects.
The MS diagnosis was determined according to the 2010 revised McDonald criteria  and the NMO diagnosis was based on the revised diagnostic criteria for NMO . The interviews, neurological examinations and EDSS scores of the MS and NMO groups were conducted in an MS cohort study. All the NMO patients had optica neuritis and myelitis and met at least 2 of the 3 supporting criteria (Brain MRI-, AQP4-IgG+, negative for MS).
Plasma chemokine and cytokines levels
To exclude the effect of different time points and other factors on the level of chemokines and cytokines, all blood samples (2 mL each) were obtained at 9:00 a.m using disposable Ethylenediaminetetraacetic acid (EDTA) vacuum blood collection tubes (BD, USA) and tested over 8 h. After 2 h of standing at 4 °C, the supernatant was pipetted into EP tubes and stored at −80 °C. Plasma ENA 78, IL-1β and TNF-α were measured using MILLIPLEX® map Human High Sensitivity Cytokine/Chemokine Panels (Cat. No. HCYP2MAG-62 K; Cat. No. HCYTOMAG-60 K), according to the manufacturer’s instructions.
Statistical analysis was performed using GraphPad Prism version 5 (GraphPad Software, Inc., California) and the data were reported as Means ± SEM. Mann–Whitney U and Kruskal-Wallis tests were used to compare 2 to 3 groups, respectively. Pearson’s test was used to perform correlations. A P value of < 0.05 was considered statistically significant.
Demographic and clinical data of HC, MS and NMO
HC (n = 20)
MS (n = 25)
NMO (n = 25)
Mean ± SE
32.30 ± 1.80
33.84 ± 2.31
36.80 ± 2.45
Age at onset (year)
Mean ± SE
29.84 ± 2.28
30.24 ± 2.34
Disease duration (year)
Mean ± SE
3.92 ± 0.53
6.04 ± 1.01
Mean ± SE
1.61 ± 0.47
1.69 ± 0.47
Mean ± SE
1.92 ± 0.28
3.24 ± 0.35*
Increased plasma ENA 78, IL-1β and TNF-α levels in NMO patients
Correlation between ENA 78 gradients and EDSS scores in the MS and NMO groups
The perivascular presence of neutrophils is one of the primary histological differences between MS and NMO, as reported in NMO patients as well as in mouse and rat models [2, 7, 26]. Neutrophils are elevated about 20 % in the CSF during remission of NMO patients . In mouse models of NMO, tissue damage was ameliorated by eliminating neutrophils, whereas increased neutrophils exacerbated tissue damage [7, 28]. ENA 78 is one of the ELR+ chemokines specifically inducing neutrophil migration, with the ability to interact with chemokine receptors CXCR1 and CXCR2 [29, 30]. ENA 78 stimulates neutrophil directed chemotaxis by promoting the intracellular level of elastase and free calcium and inducing neutrophil pro-adhesive activity [31, 32]. In addition, inhibitors of neutrophil elastase, which are involved in neutrophil migration and neutrophil-mediated tissue damage, have been tested in experimental trials such as Sivelestat [7, 33–35]. Other studies also indicated that the increased ENA 78 amplified the pro-inflammatory cytokine response, which had a direct chemo-attracting effect on neutrophils [36–39]. Therefore, we studied ENA 78 and found that it was dramatically increased in the NMO patients (vs. HC, P < 0.001; vs. MS, P < 0.05). Studies proved that ENA 78 was detected in eosinophils, which also aggregated in the NMO lesions [2, 8, 40], suggesting that eosinophils recruit and activate CXCR2+ cells such as neutrophils by secreting ENA 78. In the present study, we found that the plasma ENA 78 gradient was correlated with EDSS in NMO patients rather than in MS (P < 0.05). ENA 78 causes neutrophil aggregation and hyperactivation around the lesions in NMO resulting in demyelination, which is different from the pathophysiological mechanisms of MS. The higher ENA 78 gradient in the blood leads to increased neutrophil aggregation around the lesions, causing severe clinical symptoms.
Although the precise mechanism involving ENA 78 upregulation is not fully understood, factors involved in modulating ENA 78 expression at the transcriptional level and signaling pathways are known in different types of cells [41–43]. Neutrophil chemoattractant chemokines belonging to ELR-CXCL family, especially ENA 78 binding with chemokine receptor CXCR2, mediate the IL-1β driven cell recruitment [43, 44]. ELR+ chemokines, including CXCL1, CXCL2 and ENA 78, are triggered by IL-1β [45–47]. IL-1β inducing ENA 78 expression by activating cAMP-response element binding protein (CREB) and NF-kB promoter of ENA 78 is part of the inflammatory response in vitro and in vivo [39, 43, 48–50]. IL-1β induces leukocyte rolling, adherence and emigration associated with an increase in kinin B1 receptor mRNA expression, which plays a role in neutrophil recruitment . This current study results showed that increased IL-1β levels in NMO patients matched the higher ENA 78 levels in the periphery compared with the HC (P < 0.01). TNF-α, which induces neutrophil influx, exacerbates the lesions in ex vivo spinal cord and optical nerve of NMO [28, 51]. Our findings, herein, show that NMO and MS patients had higher plasma levels of TNF-α compared with HC (P < 0.001; P < 0.05, respectively). Further, TNF-α potentially increases the adhesion-molecule expression in the brain suggesting a role for anti-TNF therapies in NMO [8, 52, 53]. The overexpression of IL-1β and TNF-α might be one of the factors inducing severe lesions in NMO, exacerbating the damage mediated by higher ENA 78 levels.
In summary, the overproduction of pro-inflammatory cytokines such as IL-1β and TNF-α during remission of NMO might result in activation of ENA 78. High levels of ENA 78 may play a critical role in neutrophil infiltration during NMO inflammation. And these might lead to increased neutrophils aggregation around the lesion, causing severer clinical symptoms once NMO relapse. ENA 78 plasma levels were also correlated with EDSS scores in NMO remission. The current study enables the therapy of NMO patients.
AQP, aquaporin; CREB, cAMP-response element binding protein; CNS, central nervous system; CXCL, chemokine (C-X-C motif) ligand; NMO, Neuromyelitis optica; NMO, neuromyelitis optica, ENA, epithelial neutrophil-activating peptide, EDTA, Ethylenediaminetetraacetic acid, MS, multiple sclerosis; HC, healthy controls; EDSS, expanded disability status score; IL-1β, interleukin 1β; TNF-α, tumor necrosis factor α; PMNLs, polymorphonuclear leukocytes; ELR, glutamic acid-leucine-arginine motif
We thank Hui Wang, Yuezhi Kang and Cuicui Cheng who collected blood from patients and health subjects and thereby made it possible to carry out the study.
Yongping Fan, Lei Wang were responsible for accessing research funds: National Natural Science Foundation of China (CN) (81072765, 81173237, 81473640, 81273742); Natural Science Foundation of Beijing Municipality (CN) (7142053).
Availability of data and materials
All the data supporting our findings is contained within the manuscript.
TY, SW, LW carried out the MILLIPLEX® map Human High Sensitivity Cytokine/Chemokine Panels tests, participated in the collection of samples and drafted the manuscript. QZ, QL, MW and ZD performed the statistical analysis and valued EDSS scores of patients. TY, SW and YF participated in the design of the study and coordination. TY conceived of the study, and participated in its design. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
The study protocol was approved by the local ethics committee (IRB of Beijing Tiantan Hospital Affiliated to Capital Medical University, No. KY2015-003-02). Written informed consent was obtained from all participants.
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- Wingerchuk DM, Hogancamp WF, O’Brien PC, Weinshenker BG. The clinical course of neuromyelitis optica (Devic’s syndrome). Neurology. 1999;53:1107–14.View ArticlePubMedGoogle Scholar
- Lucchinetti CF, Mandler RN, McGavern D, Bruck W, Gleich G, Ransohoff RM, et al. A role for humoral mechanisms in the pathogenesis of Devic’s neuromyelitis optica. Brain. 2002;125:1450–61.View ArticlePubMedGoogle Scholar
- Asgari N, Owens T, Frokiaer J, Stenager E, Lillevang ST, Kyvik KO. Neuromyelitis optica (NMO)--an autoimmune disease of the central nervous system (CNS). Acta Neurol Scand. 2011;123:369–84.View ArticlePubMedGoogle Scholar
- Shiee N, Bazin PL, Zackowski KM, Farrell SK, Harrison DM, Newsome SD, et al. Revisiting brain atrophy and its relationship to disability in multiple sclerosis. PLoS One. 2012;7:e37049.View ArticlePubMedPubMed CentralGoogle Scholar
- Confavreux C, Vukusic S. The clinical epidemiology of multiple sclerosis. Neuroimaging Clin N Am. 2008;18:589–622. ix-x.View ArticlePubMedGoogle Scholar
- Amato MP, Goretti B, Ghezzi A, Lori S, Zipoli V, Moiola L, et al. Cognitive and psychosocial features in childhood and juvenile MS: two-year follow-up. Neurology. 2010;75:1134–40.View ArticlePubMedGoogle Scholar
- Saadoun S, Waters P, MacDonald C, Bell BA, Vincent A, Verkman AS, et al. Neutrophil protease inhibition reduces neuromyelitis optica-immunoglobulin G-induced damage in mouse brain. Ann Neurol. 2012;71:323–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Papadopoulos MC, Bennett JL, Verkman AS. Treatment of neuromyelitis optica: state-of-the-art and emerging therapies. Nat Rev Neurol. 2014;10:493–506.View ArticlePubMedPubMed CentralGoogle Scholar
- Steinman L, Zamvil SS. How to successfully apply animal studies in experimental allergic encephalomyelitis to research on multiple sclerosis. Ann Neurol. 2006;60:12–21.View ArticlePubMedGoogle Scholar
- Lennon VA, Wingerchuk DM, Kryzer TJ, Pittock SJ, Lucchinetti CF, Fujihara K, et al. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet. 2004;364:2106–12.View ArticlePubMedGoogle Scholar
- Rowland KJ, Diaz-Miron J, Guo J, Erwin CR, Mei J, Worthen GS, et al. CXCL5 is required for angiogenesis, but not structural adaptation after small bowel resection. J Pediatr Surg. 2014;49:976–80. discussion 980.View ArticlePubMedPubMed CentralGoogle Scholar
- Zheng J, Zhu X, Zhang J. CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration. Biochem Biophys Res Commun. 2014;446:18–24.View ArticlePubMedGoogle Scholar
- Sanz MJ, Kubes P. Neutrophil-active chemokines in in vivo imaging of neutrophil trafficking. Eur J Immunol. 2012;42:278–83.View ArticlePubMedGoogle Scholar
- Lira SA, Furtado GC. The biology of chemokines and their receptors. Immunol Res. 2012;54:111–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Smit JJ, Lukacs NW. A closer look at chemokines and their role in asthmatic responses. Eur J Pharmacol. 2006;533:277–88.View ArticlePubMedGoogle Scholar
- Zwijnenburg PJ, de Bie HM, Roord JJ, van der Poll T, van Furth AM. Chemotactic activity of CXCL5 in cerebrospinal fluid of children with bacterial meningitis. J Neuroimmunol. 2003;145:148–53.View ArticlePubMedGoogle Scholar
- Kawamura M, Toiyama Y, Tanaka K, Saigusa S, Okugawa Y, Hiro J, et al. CXCL5, a promoter of cell proliferation, migration and invasion, is a novel serum prognostic marker in patients with colorectal cancer. Eur J Cancer. 2012;48:2244–51.View ArticlePubMedGoogle Scholar
- Sundaram K, Rao DS, Ries WL, Reddy SV. CXCL5 stimulation of RANK ligand expression in Paget’s disease of bone. Lab Invest. 2013;93:472–9.View ArticlePubMedGoogle Scholar
- Mostafa GA, Al-Ayadhi LY. The possible link between elevated serum levels of epithelial cell-derived neutrophil-activating peptide-78 (ENA-78/CXCL5) and autoimmunity in autistic children. Behav Brain Funct. 2015;11:11.View ArticlePubMedPubMed CentralGoogle Scholar
- Gillitzer R, Ritter U, Spandau U, Goebeler M, Bröcker EB. Differential expression of GRO-alpha and IL-8 mRNA in psoriasis: a model for neutrophil migration and accumulation in vivo. J Invest Dermatol. 1996;107:778–82.View ArticlePubMedGoogle Scholar
- Stillie R, Farooq SM, Gordon JR, Stadnyk AW. The functional significance behind expressing two IL-8 receptor types on PMN. J Leukoc Biol. 2009;86:529–43.View ArticlePubMedGoogle Scholar
- Rocha BC, Marques PE, Leoratti FM, Junqueira C, Pereira DB, Antonelli LR, et al. Type I Interferon Transcriptional Signature in Neutrophils and Low-Density Granulocytes Are Associated with Tissue Damage in Malaria. Cell Rep. 2015;13:2829–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Deng M, Ma T, Yan Z, Zettel KR, Scott MJ, Liao H, et al. Toll-like Receptor 4 Signaling on Dendritic Cells Suppresses Polymorphonuclear Leukocyte CXCR2 Expression and Trafficking via Interleukin 10 During Intra-abdominal Sepsis. J Infect Dis. 2015;213(8):1280–8.View ArticlePubMedGoogle Scholar
- Polman CH, Reingold SC, Banwell B, Clanet M, Cohen JA, Filippi M, et al. Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol. 2011;69:292–302.View ArticlePubMedPubMed CentralGoogle Scholar
- Wingerchuk DM, Lennon VA, Pittock SJ, Lucchinetti CF, Weinshenker BG. Revised diagnostic criteria for neuromyelitis optica. Neurology. 2006;66:1485–9.View ArticlePubMedGoogle Scholar
- Saadoun S, Waters P, Bell BA, Vincent A, Verkman AS, Papadopoulos MC. Intra-cerebral injection of neuromyelitis optica immunoglobulin G and human complement produces neuromyelitis optica lesions in mice. Brain. 2010;133:349–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Jarius S, Paul F, Franciotta D, Ruprecht K, Ringelstein M, Bergamaschi R, et al. Cerebrospinal fluid findings in aquaporin-4 antibody positive neuromyelitis optica: results from 211 lumbar punctures. J Neurol Sci. 2011;306:82–90.View ArticlePubMedGoogle Scholar
- Zhang H, Bennett JL, Verkman AS. Ex vivo spinal cord slice model of neuromyelitis optica reveals novel immunopathogenic mechanisms. Ann Neurol. 2011;70:943–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Tsai HH, Frost E, To V, Robinson S, Ffrench-Constant C, Geertman R, et al. The chemokine receptor CXCR2 controls positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. Cell. 2002;110:373–83.View ArticlePubMedGoogle Scholar
- Pelus LM, Fukuda S. Peripheral blood stem cell mobilization: the CXCR2 ligand GRObeta rapidly mobilizes hematopoietic stem cells with enhanced engraftment properties. Exp Hematol. 2006;34:1010–20.View ArticlePubMedGoogle Scholar
- Zineh I, Aquilante CL, Langaee TY, Beitelshees AL, Arant CB, Wessel TR, et al. CXCL5 gene polymorphisms are related to systemic concentrations and leukocyte production of epithelial neutrophil-activating peptide (ENA-78). Cytokine. 2006;33:258–63.View ArticlePubMedGoogle Scholar
- Zineh I, Luo X, Welder GJ, Debella AE, Wessel TR, Arant CB, et al. Modulatory effects of atorvastatin on endothelial cell-derived chemokines, cytokines, and angiogenic factors. Pharmacotherapy. 2006;26:333–40.PubMedGoogle Scholar
- Young RE, Thompson RD, Larbi KY, La M, Roberts CE, Shapiro SD, et al. Neutrophil elastase (NE)-deficient mice demonstrate a nonredundant role for NE in neutrophil migration, generation of proinflammatory mediators, and phagocytosis in response to zymosan particles in vivo. J Immunol. 2004;172:4493–502.View ArticlePubMedGoogle Scholar
- Papayannopoulos V, Metzler KD, Hakkim A, Zychlinsky A. Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J Cell Biol. 2010;191:677–91.View ArticlePubMedPubMed CentralGoogle Scholar
- Iwata K, Doi A, Ohji G, Oka H, Oba Y, Takimoto K, et al. Effect of neutrophil elastase inhibitor (sivelestat sodium) in the treatment of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS): a systematic review and meta-analysis. Intern Med. 2010;49:2423–32.View ArticlePubMedGoogle Scholar
- Lin M, Carlson E, Diaconu E, Pearlman E. CXCL1/KC and CXCL5/LIX are selectively produced by corneal fibroblasts and mediate neutrophil infiltration to the corneal stroma in LPS keratitis. J Leukoc Biol. 2007;81:786–92.View ArticlePubMedGoogle Scholar
- Hosking MP, Liu L, Ransohoff RM, Lane TE. A protective role for ELR+ chemokines during acute viral encephalomyelitis. PLoS Pathog. 2009;5:e1000648.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou SL, Dai Z, Zhou ZJ, Wang XY, Yang GH, Wang Z, et al. Overexpression of CXCL5 mediates neutrophil infiltration and indicates poor prognosis for hepatocellular carcinoma. Hepatology. 2012;56:2242–54.View ArticlePubMedGoogle Scholar
- Guha D, Klamar CR, Reinhart T, Ayyavoo V. Transcriptional Regulation of CXCL5 in HIV-1-Infected Macrophages and Its Functional Consequences on CNS Pathology. J Interferon Cytokine Res. 2015;35:373–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Persson T, Monsef N, Andersson P, Bjartell A, Malm J, Calafat J, et al. Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils. Clin Exp Allergy. 2003;33:531–7.View ArticlePubMedGoogle Scholar
- Vieira SM, Lemos HP, Grespan R, Napimoga MH, Dal-Secco D, Freitas A, et al. A crucial role for TNF-alpha in mediating neutrophil influx induced by endogenously generated or exogenous chemokines, KC/CXCL1 and LIX/CXCL5. Br J Pharmacol. 2009;158:779–89.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang H, Yang R, Wang Z, Lin G, Lue TF, Lin CS. Adipose tissue-derived stem cells secrete CXCL5 cytokine with neurotrophic effects on cavernous nerve regeneration. J Sex Med. 2011;8:437–46.View ArticlePubMedGoogle Scholar
- Chandrasekar B, Melby PC, Sarau HM, Raveendran M, Perla RP, Marelli-Berg FM, et al. Chemokine-cytokine cross-talk. The ELR+ CXC chemokine LIX (CXCL5) amplifies a proinflammatory cytokine response via a phosphatidylinositol 3-kinase-NF-kappa B pathway. J Biol Chem. 2003;278:4675–86.View ArticlePubMedGoogle Scholar
- Duchene J, Lecomte F, Ahmed S, Cayla C, Pesquero J, Bader M, et al. A novel inflammatory pathway involved in leukocyte recruitment: role for the kinin B1 receptor and the chemokine CXCL5. J Immunol. 2007;179:4849–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Bersinger NA, Gunthert AR, McKinnon B, Johann S, Mueller MD. Dose–response effect of interleukin (IL)-1beta, tumour necrosis factor (TNF)-alpha, and interferon-gamma on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells. Arch Gynecol Obstet. 2011;283:1291–6.View ArticlePubMedGoogle Scholar
- Carrero R, Cerrada I, Lledo E, Dopazo J, Garcia-Garcia F, Rubio MP, et al. IL1beta induces mesenchymal stem cells migration and leucocyte chemotaxis through NF-kappaB. Stem Cell Rev. 2012;8:905–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Okabe H, Beppu T, Ueda M, Hayashi H, Ishiko T, Masuda T, et al. Identification of CXCL5/ENA-78 as a factor involved in the interaction between cholangiocarcinoma cells and cancer-associated fibroblasts. Int J Cancer. 2012;131:2234–41.View ArticlePubMedGoogle Scholar
- Sun H, Chung WC, Ryu SH, Ju Z, Tran HT, Kim E, et al. Cyclic AMP-responsive element binding protein- and nuclear factor-kappaB-regulated CXC chemokine gene expression in lung carcinogenesis. Cancer Prev Res (Phila). 2008;1:316–28.View ArticleGoogle Scholar
- Mamik MK, Banerjee S, Walseth TF, Hirte R, Tang L, Borgmann K, et al. HIV-1 and IL-1beta regulate astrocytic CD38 through mitogen-activated protein kinases and nuclear factor-kappaB signaling mechanisms. J Neuroinflammation. 2011;8:145.View ArticlePubMedPubMed CentralGoogle Scholar
- Ye L, Huang Y, Zhao L, Li Y, Sun L, Zhou Y, et al. IL-1beta and TNF-alpha induce neurotoxicity through glutamate production: a potential role for neuronal glutaminase. J Neurochem. 2013;125:897–908.View ArticlePubMedPubMed CentralGoogle Scholar
- Tessier PA, Naccache PH, Clark-Lewis I, Gladue RP, Neote KS, McColl SR. Chemokine networks in vivo: involvement of C-X-C and C-C chemokines in neutrophil extravasation in vivo in response to TNF-alpha. J Immunol. 1997;159:3595–602.PubMedGoogle Scholar
- Zhou H, Lapointe BM, Clark SR, Zbytnuik L, Kubes P. A requirement for microglial TLR4 in leukocyte recruitment into brain in response to lipopolysaccharide. J Immunol. 2006;177:8103–10.View ArticlePubMedGoogle Scholar
- Kim S, Steelman AJ, Koito H, Li J. Astrocytes promote TNF-mediated toxicity to oligodendrocyte precursors. J Neurochem. 2011;116:53–66.View ArticlePubMedGoogle Scholar