The relevance of cortical lesions in patients with multiple sclerosis
© The Author(s). 2016
Received: 5 January 2016
Accepted: 12 October 2016
Published: 21 October 2016
Recent studies suggest that cortical lesions in multiple sclerosis (MS) substantially contribute to clinical disease severity. The present study aimed at investigating clinical, neuroanatomical, and cognitive correlates of these cortical lesions with a novel approach, i.e. by comparing two samples of relapsing-remitting multiple sclerosis (RRMS) patients, one group with and the other without cortical lesions.
High-resolution structural MRI was acquired from 42 RRMS patients and 43 controls (HC). The patient group was dichotomized based on the presence versus absence of DIR-hyperintense cortex-involving lesions, resulting in a cortical lesion group (CL, n = 32) and a non-cortical lesion group (nCL, n =10). Cognitive functioning was assessed in all participants with a comprehensive neuropsychological battery, covering mnestic, executive, and attentional functions.
Highest densities of cortical lesions in the CL group were observed in the bilateral parahippocampal gyrus. Relative to HC, patients with cortical lesions - but not those without - showed significant global cortical thinning and mnestic deficits. The two patient groups did not differ from each other regarding demographic and basic disease characteristics such as EDSS scores.
The appearance of cortical lesions in MS patients is associated with cortical thinning as well as mnestic deficits, which might be key characteristics of a 'cortically dominant' MS subtype.
KeywordsMultiple sclerosis Cortical lesions Memory
Traditionally, multiple sclerosis (MS) is considered as an autoimmune inflammatory disorder , predominantly affecting the white matter of the central nervous system . This view is based on the high sensitivity of conventional MRI sequences in the detection of white matter abnormalities in MS patients. Nevertheless, histopathologic analyses of demyelinated foci in the cerebral cortex of patients with MS - dating back to the beginning of the 20th century  - gave first evidence for cortical involvement in MS. In the early 1960s, Brownell and Hughes described that 26 % of the MS lesions affected the gray matter . However, the classical view of MS as a pure white matter pathology has not been overcome until the beginning of the 21st century. Due to the introduction of more sensitive imaging techniques such as double inversion recovery (DIR), gray matter involvement of the MS pathology is now well established [5, 6]. By suppressing the signals form the cerebrospinal fluid and white matter, DIR sequences have a higher sensitivity compared to conventional MR sequences  and thus, have made a major contribution in detecting focal cortical lesions in MS. Although a recent study has shown that DIR can detect only a minority of cortical lesions in MS, the same study has also shown a significant correlation between the number of DIR-hyperintense cortical lesions on the one hand and the total number of cortical lesions in post-mortem histopathologic analysis on the other hand . Moreover, DIR is currently considered the best wide-scale application pulse sequence for cortical lesion detection and offers high sensitivity, specificity and accuracy for the detection of gray matter lesions .
Multiple sclerosis is associated with a variety of symptoms that are responsible for functional impairment in affected patients. Besides physical disability, cognitive deficits – which were firstly described in the second half of the 19th century  – are found in up to 70 % of patients with multiple sclerosis at both the earlier and later stages of the disease . MS-related cognitive impairment can affect various aspects of cognition. Processing speed and episodic memory seem most commonly affected . However, MS patients often exhibit significant deficits in executive functions too [12, 13].
The extended focus in MS research including gray matter pathology had important consequences for the study of the neural correlates of MS symptoms. In recent years, the contribution of DIR-hyperintense cortical lesions to functional loss is particularly highlighted. Cross-sectional and longitudinal studies have shown correlations between the number and/or volume of cortical lesions and cognitive [14, 15] or physical  impairment. Regional associations are also described, for instance between an accumulation of cortical lesions in mesio-temporal areas and impairments in episodic memory [12, 17].
However, these studies are not without limitations. For example, detailed cognitive investigation  or DIR sequences were lacking . Here we try to overcome these shortcomings and apply a novel methodological approach to investigate the clinical relevance of cortical lesions in MS, i.e. by dichotomizing patients based on the presence or absence of cortical lesions. The resulting two subgroups were compared with regard to demography, cognition, fatigue, affective mood state, and several other established MRI markers of disease severity, for example T2 lesion load, third ventricle width, and global cortical thinning.
Forty-two patients with a diagnosis of relapsing-remitting multiple sclerosis (RRMS) according to the McDonald 2010 criteria  were recruited at the Multiple Sclerosis Centre of the University Hospital of Zurich. Patients with at least one DIR-hyperintense cortex-involving lesion were assigned to the cortical lesion group (CL group, n = 32), the remaining patients formed the non-cortical lesion group (nCL group, n = 10). All patients received immunomodulatory treatment - 30 with natalizumab, seven with beta-interferons, three with fingolimod, one with glatiramer acetat and one with dimethylfumarate. Exclusion criteria were a relapse or steroid-treatment during the last two months, current or past neurological disorders in addition to multiple sclerosis, and psychiatric disorders apart from MS-related depressive mood state. Moreover, none of the patients was affected by severe visual deficits or upper limb sensorimotor impairment that could hinder cognitive test performance. Furthermore, 43 age-, gender-, handedness- and education-matched healthy control persons (HC) without previous or present history of neurological or psychiatric diseases were included. Controls received financial compensation for their attendance.
MRI data acquisition
The MR scan was performed within one month of the neurological and neuropsychological examinations. All images were acquired using a 1.5-T scanner (Siemens Magnetom Avanto™) equipped with a SQ-engine gradient (45 m/T/m @ 200 T/m/s), using a dedicated 32-channel head coil. No hardware upgrades of the scanner occurred during the study period. The following sequences were performed in all subjects: (1) 3D Double Inversion Recovery (DIR) (voxel size = 1.5 x 1.5 x 1.5 mm, slice thickness = 1.5 mm, repetition time = 7500 ms, echo time = 308 ms, inversion timing 1 = 3000 ms, inversion timing 2 = 450 ms); (2) 3D T1-weighted MPRAGE (voxel size = 1 x 1 x 1 mm, slice thickness = 1 mm, repetition time = 2420 ms, echo time = 4.18 ms), and (3) 3D FLAIR (voxel size = 0.9 x 0.9 x 2.0 mm, slice thickness = 2 mm, repetition time = 5000 ms, echo time = 342 ms, inversion time = 1800 ms).
MRI post-processing and statistical analysis
The classification of cortical lesions was conducted according to the consensus recommendations of Geurts and colleagues . Consequently, cortical lesions were defined as those lesions appearing hyperintense on DIR images compared to surrounding normal-appearing gray matter, entirely or partly located in the cortical gray matter and occupying at least three voxels. Juxtacortical lesions (lesions not entering, but neighboring the cortical mantle) were not scored. DIR-hyperintense lesions were identified and manually delineated with MRIcron (http://people.cas.sc.edu/rorden/mricron/index.html), which was further used to measure total cortical lesion volume. An experienced rater, supervised by a neuroradiologist, assessed all images. The same procedure was applied to FLAIR images in order to identify FLAIR-hyperintense lesions. We used Statistical Parametric Mapping (SPM8, http://www.fil.ion.ucl.ac.uk/spm/) to co-register and normalize the individual lesion maps to MNI (Montreal Neurological Institute) standard space according to the normalization procedure proposed by Crinion and colleagues . Normalized binary lesion maps were than plotted with MRIcron onto a T1-weighted MNI template brain to create lesion overlap plots. Central brain atrophy was examined by measuring the width of the third ventricle (TVW) according to the procedure proposed by Benedict and colleagues . Brain parenchymal fraction (BPF), calculated as the ratio of brain parenchymal tissue volume to the total intracranial volume, was used as a measurement of whole-brain atrophy. The calculation was performed with Jim software (Xinapse Systems Ltd., Northants (UK); http://www.xinapse.com). Global cortical thickness evaluation was performed with the semi-automated Freesurfer image analysis suite based on MPRAGE images, which is documented and freely available online (https://surfer.nmr.mgh.harvard.edu/). Further information and technical details of these procedures are described in prior publications [22, 23]. To detect possible misclassifications of white and gray matter due to multiple sclerosis lesions, all images were visually inspected after the white/gray matter segmentation. In two patients, a semi-automated correction of topological defects was required. We used the manual procedure of control points, which is implemented in the Freesurfer software package. No further lesion masking was needed in order to obtain accurate reconstructions of the pial and the white matter surfaces.
Clinical and neuropsychological assessment
All patients underwent neurological status examination, including the Expanded Disability Status Scale (EDSS). Cognitive functions were assessed with a comprehensive battery of validated and standardized neuropsychological tests. To minimize the issue of multiple statistical testing, composite index scores were computed for mnestic, executive and attentional functions by averaging z-scores for all subtests of the corresponding function . An overview of the indices is shown in Additional file 1. The mnestic index score was derived from the delayed free recall and recognition of a 15-item word list  as well as the delayed free recall and recognition of a previously copied complex geometric figure . Phonemic-verbal  and figural fluency , response inhibition  and cognitive flexibility  formed the executive index. The attentional index was based on processing speed during color naming  as well as on reaction times from tasks measuring alertness and selective attention . Moreover, participants had to complete a German version  of the CES-D Depression questionnaire  and the Würzburg Fatigue Inventory (WEIMuS)  to self-rate depressive symptoms as well as fatigue during the last week. Similar to other studies , cognitive reserve was examined with passive vocabulary knowledge (multiple choice word test) .
Statistical analyses were performed with SPSS (IBM, Chicago, USA, Version, 21.0, https://www.ibm.com/marketplace/cloud/statistical-analysis-and-reporting). Unless otherwise stated, a p-level below 5 % was considered statistically significant. Assumptions for normality were tested for all continuous data with Kolmogorov-Smirnov tests. In case of normally distributed variables, a multivariate ANOVA was used to compare groups. Bonferroni correction was applied for post-hoc analyses. When variables were not normally distributed, non-parametric tests were applied (Mann–Whitney U, Kruskal-Wallis).
Demographic and clinical variables
Demographic and disease characteristics of multiple sclerosis patients and healthy controls
nCL group (n = 10), Mean (SD)
CL group (n = 32), Mean (SD)
Controls (n = 43), Mean (SD)
Age at diagnosis, years
Disease duration, years
Cognitive test results, fatigue and depression questionnaires
The impact of gray matter pathology, in particular that of gray matter lesions, on cognitive and physical functioning in MS patients has been discussed for many years. Here we highlight the clinical relevance of DIR-hyperintense cortical lesions in patients with RRMS. The most intriguing finding of the present study was that patients with - compared to those without visible cortical lesions - differed from each other in global cortical thickness and mnestic functions, whereas no differences between these two patient groups were observed regarding EDSS, age, age at diagnosis, disease duration, or non-mnestic cognitive functions. Moreover, patients without cortical lesions showed normal cortical thickness and mnestic functions, when compared with a group of healthy controls.
That a reduction of cortical thickness can occur in MS  - and that this thinning is related to global e.g.  and even specific cognitive impairment [12, 38] - has been found in previous studies. Similar to a recent finding , we show an association between MS-related cortical thinning and the presence of cortical MS lesions. It is known that gray matter pathology involves both inflammatory and degenerative mechanisms, but the relationship between the two remains unclear . Gray matter atrophy might be the final step of several pathological processes, which could include cortical demyelination but also retrograde degeneration secondary to white matter lesions and, perhaps, primary neurodegeneration .
We detected DIR-hyperintense cortical lesions in 76 % of our RRMS patients, supporting the notion of a high prevalence of these lesions . In further agreement with previous findings , we observed an uneven spatial distribution of cortical lesions over the cerebral cortex, with a prominent accumulation in memory-relevant mesiotemporal regions, particularly in the bilateral parahippocampal gyrus. While the importance of the hippocampus for memory function is known since the classical description of the patient H.M. in 1957 , parahippocampal involvement in memory functions was recognized only two decades ago . Squire and Zola-Morgen identified the anatomical components of what is termed the medial temporal memory system . By now it is well known that bilateral damage to the medial temporal lobe causes severe learning and memory impairments. This relationship has also been shown in MS patients. Learning and memory is the most frequently disrupted cognitive domain in MS, reported in 40–60 % of patients . Moreover, Coebergh et al.  described a patient with acute memory impairment, associated with hippocampal and cortical lesions. Cortical lesions were also associated with cognitive decline in a group of 13 MS patients . In this study, a significant correlation between hippocampal lesion load and visuospatial memory was observed. Based on the present and previous findings, we thus conclude that mesiotemporal cortical lesions are highly prevalent in RRMS patients and play a crucial role in the development of mnestic dysfunction.
The association we found between memory impairment and both cortical thinning as well as cortical lesions seems particularly intriguing: One might speculate that mnestic dysfunction in MS patients could indicate cortical involvement of the MS pathology in general. Related to this assumption, a rarely occurring variant of so-called 'cortical MS' has been described in previous studies [47, 48]. The condition was predominantly characterized by the presence of neurobehavioral symptoms (e.g. depression, apathy) and neuropsychological deficits (e.g. agraphia, anomia) suggesting cortical dysfunction. However, detailed and explicit imaging data for detecting cortical involvement (e.g. DIR) is missing in these studies. By approaching from the imaging side, our patient group with cortical involvement may reflect a different and somehow incomplete variant of cortical MS, as they showed distinct mnestic deficits in association with cortical involvement, but no other cortical symptoms such for example depression. Moreover, we found no patient with cortical lesions in the absence of subcortical lesions. In all patients, including those of the CL group, the majority of MS lesions was located subcortically. Taking this into account, we here propose that our CL group may represent a 'cortically dominant' subtype of MS. In these patients, pathophysiological processes might be different from those of patients without cortical involvement.
Beside the difference between the two patient groups in mnestic functions, both patient group showed executive deficits compared to the healthy control group. This finding is in line with previous studies that have shown significant executive dysfunction in MS patients [12, 13, 49].
Much research and clinical development in MS has focused on the inflammatory mechanisms of the disease. Meanwhile, multiple disease-modifying drugs (DMD) are available that target the inflammatory pathology of MS, in particular the development of new white matter lesions . A recent study demonstrated that DMD - in particular IFN β-1a and glatiramer acetate - can reduce the accumulation of cortical lesions too . In addition, Filippi and colleagues  showed that the presence of at least one cortical lesion is associated with a high risk of conversion from clinically isolated syndrome (CIS) to definite MS within a short period. Together with our results, these findings highlight the relevance of cortical lesions as a “target” in the development of new DMD, and to include cortical lesions as a primary outcome variable in disease and treatment monitoring.
This study is not without limitations. As already mentioned earlier, DIR represents only a limited snapshot of the real cortical pathology that is present in MS patients. The combination of DIR and a T1-weighted phase-sensitive inversion recovery (PSIR) sequence would substantially improve the sensitivity of detecting lesions present in the cortex of MS patients . Moreover, the present analyses are limited due to the low MRI field (1.5 T) applied. It has been shown that high field (3 T) and ultra-high field (7 T) MRI systems deliver a higher detection rate of cortical lesions in vivo . Finally, a further limitation of the present study is the relatively small and unbalanced sample size.
In conclusion, the occurrence of cortical lesions in MS is clinically relevant insofar as it is associated with neurodegenerative cortical thinning and mnestic dysfunction. Although with today’s imaging techniques, it is only possible to visualize the 'tip of the iceberg' of cortical MS lesions [7, 55], further progress in detection algorithms can be expected and will likely improve our understanding of MS pathology, symptoms, and treatment.
Brain parenchymal fraction
Cortical lesion group
Double inversion recovery
Expanded disability status scale
Magnetic resonance imaging
Non-cortical lesion group
Relapsing-remitting multiple sclerosis
Third ventricle width
This study was supported by the Swiss Multiple Sclerosis Society and Bayer Switzerland.
Availability of data and materials
The data set on which the conclusion of the paper rely cannot be deposited in a publicly available repositories or shared due to confidentiality restrictions on data obtained from the local ethical committee.
OG drafted the manuscript for content, contributed to the analysis/interpretation of the data, performed statistical analyses and neuropsychological assessments. TP contributed to the study concept and design, the analysis/interpretation of the data and to the drafting of the manuscript for content and performed neuropsychological assessments. LB contributed to data analysis/interpretation and to revising the manuscript. KR contributed to data analysis and performed clinical evaluations. DW contributed to the data analysis. BS assisted in the analysis of data and acquisition of data. PB assisted in interpretation of data and revising the manuscript. ML contributed to the study concept/design, obtaining funding and revising the manuscript. He also acted as study supervisor. All authors read and approved the final version of the manuscript.
ML received grants, funding or honoraria from Bayer, Biogen, Genzyme, Merck, Novartis and Teva. The other authors report no disclosures.
Consent for publication
Ethics approval and consent to participate
The study was approved by the local ethics committee, and written informed consent was obtained from all subjects.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Sospedra M, Martin R. Immunology of multiple sclerosis. Annu Rev Immunol. 2005;23:683–747.View ArticlePubMedGoogle Scholar
- Rao SM. White Matter Disease and Dementia. Brain Cogn. 1996;31:250–68.View ArticlePubMedGoogle Scholar
- Dawson J. The histology of multiple sclerosis. Trans R Soc Edinburgh. 1916;5:517–740.View ArticleGoogle Scholar
- Brownell B, Hughes JT. The distribution of plaques in the cerebrum in multiple sclerosis. J Neurol Neurosurg Psychiatry. 1962;25:315–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Geurts J, Barkhof F. Grey matter pathology in multiple sclerosis. Lancet Neurol. 2008;7:841–51.View ArticlePubMedGoogle Scholar
- Calabrese M, Magliozzi R, Ciccarelli O, Geurts JJG, Reynolds R, Martin R. Exploring the origins of grey matter damage in multiple sclerosis. Nat Rev Neurosci. 2015;16:147–58.View ArticlePubMedGoogle Scholar
- Seewann A, Vrenken H, Kooi E-J, van der Valk P, Knol DL, Polman CH, et al. Imaging the tip of the iceberg: visualization of cortical lesions in multiple sclerosis. Mult Scler. 2011;17:1202–10.View ArticlePubMedGoogle Scholar
- Calabrese M, De Stefano N. Cortical lesion counts by double inversion recovery should be part of the MRI monitoring process for all MS patients: Yes. Mult Scler J. 2014;20:537–8.View ArticleGoogle Scholar
- Charcot J-M. Leçons sur les maladies du système nerveux. London: New Sydenham Society; 1877.Google Scholar
- Pflugshaupt T, Geisseler O, Nyffeler T, Linnebank M. Cognitive impairment in Multiple Sclerosis: Clinical Manifestation, Neuroimaging Correlates, and Treatment. Semin Neurol. 2016 (in press)
- Chiaravalloti N, DeLuca J. Cognitive impairment in multiple sclerosis. Lancet Neurol. 2008;7:1139–51.View ArticlePubMedGoogle Scholar
- Geisseler O, Pflugshaupt T, Bezzola L, Reuter K, Weller D, Schuknecht B, et al. Cortical thinning in the anterior cingulate cortex predicts multiple sclerosis patients’ fluency performance in a lateralised manner. NeuroImage Clin. 2016;10:89–95.View ArticlePubMedGoogle Scholar
- Drew M, Tippett L, Starkey N, Isler R. Executive dysfunction and cognitive impairment in a large community-based sample with multiple sclerosis from New Zealand: a descriptive study. Arch Clin Neuropsychol. 2008;23:1–19.View ArticlePubMedGoogle Scholar
- Calabrese M, Agosta F, Rinaldi F, Mattisi I, Grossi P, Favaretto A, et al. Cortical lesions and atrophy associated with cognitive impairment in relapsing-remitting multiple sclerosis. Arch Neurol. 2009;66:1144–50.View ArticlePubMedGoogle Scholar
- Mike A, Glanz BI, Hildenbrand P, Meier D, Bolden K, Liguori M, et al. Identification and Clinical Impact of Multiple Sclerosis Cortical Lesions as Assessed by Routine 3 T MR Imaging. Am J Neuroradiol. 2011;32:515–21.View ArticlePubMedGoogle Scholar
- Calabrese M, De Stefano N, Atzori M, Bernardi V, Mattisi I, Barachino L, et al. Detection of cortical inflammatory lesions by double inversion recovery magnetic resonance imaging in patients with multiple sclerosis. Arch Neurol. 2007;64:1416–22.View ArticlePubMedGoogle Scholar
- Roosendaal S, Moraal B, Pouwels P, Vrenken H, Castelijns J, Barkhof F, Geurts J, Roosendaal S, Moraal B, Pouwels P, Vrenken H, Castelijns J, Barkhof F, Geurts J. Accumulation of cortical lesions in MS: relation with cognitive impairment. Multiple Sclerosis. 2009:15(6):708–14.
- Polman CH, Reingold SC, Banwell B, Clanet M, Cohen JA, Filippi M, et al. Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol. 2011;69:292–302.View ArticlePubMedPubMed CentralGoogle Scholar
- Geurts JJG, Roosendaal SD, Calabrese M, Ciccarelli O, Agosta F, Chard DT, et al. Consensus recommendations for MS cortical lesion scoring using double inversion recovery MRI. Neurology. 2011;76:418–24.View ArticlePubMedGoogle Scholar
- Crinion J, Ashburner J, Leff A, Brett M, Price C, Friston K. Spatial normalization of lesioned brains: performance evaluation and impact on fMRI analyses. NeuroImage. 2007;37:866–75.View ArticlePubMedPubMed CentralGoogle Scholar
- Benedict R, Bruce JM, Dwyer MG, Abdelrahman N, Hussein S, Weinstock-Guttman B, et al. Neocortical atrophy, third ventricular width, and cognitive dysfunction in multiple sclerosis. Arch Neurol. 2006;63:1301–6.View ArticlePubMedGoogle Scholar
- Dale A, Fischl B, Sereno M. Cortical Surface-Based Analysis: I. Segmentation and Surface Reconstruction. NeuroImage. 1999;9:179–94.View ArticlePubMedGoogle Scholar
- Fischl B, Sereno MI, Dale A. Cortical Surface-Based Analysis: II: Inflation, Flattening, and a Surface-Based Coordinate System. NeuroImage. 1999;9:195–207.View ArticlePubMedGoogle Scholar
- La Joie R, Landeau B, Perrotin A, Bejanin A, Egret S, Pélerin A, et al. Intrinsic connectivity identifies the hippocampus as a main crossroad between Alzheimer’s and semantic dementia-targeted networks. Neuron. 2014;81:1417–28.View ArticlePubMedGoogle Scholar
- Helmstaedter C, Lendt M, Lux S. Verbaler Lern- und Merkfähigkeitstest (VLMT). Göttingen: Beltz Test; 2001.Google Scholar
- Meyers J, Meyers K. Rey complex figure test and recognition trial: Professional manual. Odessa: Psychological Assessment Resources; 1995.Google Scholar
- Aschenbrenner A, Tucha O, Lange K. Regensburger Wortflüssigkeits-Test. Handanweisung. Göttingen: Hogrefe Verlag; 2000.Google Scholar
- Haid T, Martl C, Schubert F, Wenzl M, Kofler M, Saltuari L. Der “HAMASCH 5 Punkt Test” - erste Normierungsergebnisse. Z Für Neuropsychol. 2002;13:233.Google Scholar
- Delis D, Kaplan E, Kramer J. Delis-Kaplan Executive Function System (D-KEFS). San Antonio (TX): The Psychological Corporation; 2001.Google Scholar
- Zimmermann P, Fimm B. TAP Testbatterie zur Aufmerksamkeitsprüfung. Herzogenrath: Psytest; 2007.Google Scholar
- Hautzinger M, Bailer M. Allgemeine Depressionsskala ADS. Göttingen: Beltz Test GmbH; 1992.Google Scholar
- Radloff LS. The CES-D Scale A Self-Report Depression Scale for Research in the General Population. Appl Psychol Meas. 1977;1:385–401.View ArticleGoogle Scholar
- Flachenecker P, König H, Meissner H, Müller G. Fatigue bei Multipler Sklerose: Validierung des “Würzburger Erschöpfungs-Inventar bei Multipler Sklerose (WEIMuS).”. Neuro Rehabil. 2008;14:299–306.Google Scholar
- Sumowski JF, Rocca MA, Leavitt VM, Dackovic J, Mesaros S, Drulovic J, et al. Brain reserve and cognitive reserve protect against cognitive decline over 4.5 years in MS. Neurology. 2014;82:1776–83.View ArticlePubMedPubMed CentralGoogle Scholar
- Lehrl S. Mehrfachwahl-Wortschatz-Intelligenz MWT-B. 5th ed. Balingen: Spitta Verlag; 2005.Google Scholar
- Sailer M, Fischl B, Salat D, Tempelmann C, Schonfeld M, Busa E, et al. Focal thinning of the cerebral cortex in multiple sclerosis. Brain. 2003;126:1734–44.View ArticlePubMedGoogle Scholar
- Calabrese M, Rinaldi F, Mattisi I, Grossi P, Favaretto A, Atzori M, Bernardi V, Barachino L, Romualdi C, Rinaldi L, Perini P, Gallo P. Widespread cortical thinning characterizes patients with MS with mild cognitive impairment. Neurology. 2010:74(4):321–8.
- Mike A, Strammer E, Aradi M, Orsi G, Perlaki G, Hajnal A, et al. Disconnection mechanism and regional cortical atrophy contribute to impaired processing of facial expressions and theory of mind in Multiple Sclerosis: A Structural MRI Study. PLoS ONE. 2013;8(12):e82422.
- Calabrese M, Reynolds R, Magliozzi R, Castellaro M, Morra A, Scalfari A, et al. Regional distribution and evolution of gray matter damage in different populations of multiple sclerosis patients. PLoS ONE. 2015;10:e0135428.
- Geurts JJG, Calabrese M, Fisher E, Rudick RA. Measurement and clinical effect of grey matter pathology in multiple sclerosis. Lancet Neurol. 2012;11:1082–92.View ArticlePubMedGoogle Scholar
- Calabrese M, Favaretto A, Martini V, Gallo P. Grey matter lesions in MS: from histology to clinical implications. Prion. 2013;7:20–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Scoville WB, Milner B. Loss of recent memory after bilateral hippocampal lesions. J Neurol Neurosurg Psychiatry. 1957;20:11–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Zola-Morgan S, Squire LR, Amaral DG, Suzuki WA. Lesions of perirhinal and parahippocampal cortex that spare the amygdala and hippocampal formation produce severe memory impairment. J Neurosci. 1989;9:4355–70.PubMedGoogle Scholar
- Squire LR, Zola-Morgan S. The medial temporal lobe memory system. Science. 1991;253:1380–6.View ArticlePubMedGoogle Scholar
- Rogers JM, Panegyres PK. Cognitive impairment in multiple sclerosis: evidence-based analysis and recommendations. J Clin Neurosci. 2007;14:919–27.View ArticlePubMedGoogle Scholar
- Coebergh J, Roosendaal S, Polman C, Geurts J, van Woerkom T, Coebergh J, Roosendaal S, Polman C, Geurts J, van Woerkom T. Acute severe memory impairment as a presenting symptom of multiple sclerosis: a clinical case study with 3D double inversion recovery MR imaging. Multiple Sclerosis. 2010:16(12):1521–4.
- Mojtaba Zarei, Mojtaba Zarei. Clinical characteristics of cortical multiple sclerosis. Journal of the Neurological Sciences. 2006:245(1-2):53–8.
- M Zarei, M Zarei. Cognitive presentation of multiple sclerosis: evidence for a cortical variant. Journal of Neurology, Neurosurgery & Psychiatry. 2003:74(7):872–7.
- Geisseler O, Pflugshaupt T, Buchmann A, Bezzola L, Reuter K, Schuknecht B, Weller D, Linnebank M, Brugger P. Random number generation deficits in patients with multiple sclerosis: Characteristics and neural correlates. Cortex. 2016:82:237–43.
- Barkhof F, Calabresi PA, Miller DH, Reingold SC. Imaging outcomes for neuroprotection and repair in multiple sclerosis trials. Nat Rev Neurol. 2009;5:256–66.View ArticlePubMedGoogle Scholar
- Rinaldi F, Perini P, Atzori M, Favaretto A, Seppi D, Gallo P. Disease-modifying drugs reduce cortical lesion accumulation and atrophy progression in relapsing-remitting multiple sclerosis: results from a 48-month extension study. Mult Scler Int. 2015;2015:369348.PubMedPubMed CentralGoogle Scholar
- Filippi M, Rocca MA, Calabrese M, Sormani MP, Rinaldi F, Perini P, et al. Intracortical lesions: relevance for new MRI diagnostic criteria for multiple sclerosis. Neurology. 2010;75:1988–94.View ArticlePubMedGoogle Scholar
- Nelson F, Poonawalla AH, Hou P, Huang F, Wolinsky JS, Narayana PA. Improved identification of intracortical lesions in multiple sclerosis with phase-sensitive inversion recovery in combination with fast double inversion recovery MR imaging. AJNR Am J Neuroradiol. 2007;28:1645–9.View ArticlePubMedGoogle Scholar
- Kilsdonk ID, Jonkman LE, Klaver R, van Veluw SJ, Zwanenburg JJM, Kuijer JPA, et al. Increased cortical grey matter lesion detection in multiple sclerosis with 7 T MRI: a post-mortem verification study. Brain J. Neurol. 2016;139:1472–81.
- Seewann A, Kooi E-J, Roosendaal SD, Pouwels PJW, Wattjes MP, van der Valk P, et al. Postmortem verification of MS cortical lesion detection with 3D DIR. Neurology. 2012;78:302–8.View ArticlePubMedGoogle Scholar