Injury of ascending reticular activating system associated with delayed post-hypoxic leukoencephalopathy: a case report
© The Author(s). 2017
Received: 8 May 2017
Accepted: 11 July 2017
Published: 19 July 2017
Delayed post-hypoxic leukoencephalopathy (DPHL) is a demyelinating syndrome characterized by neurological relapse after an initial recovery from hypoxic brain injury. We describe a patient with impaired consciousness following DPHL, concurrent with injury of the ascending reticular activating system (ARAS) shown using diffusion tensor tractography (DTT).
A 50-year-old male patient was in a drowsy mental state after exposure to carbon monoxide (CO) for about ten hours. About a day after the CO exposure, his mental state recovered to an alert condition. However, his consciousness deteriorated to drowsy 24 days after the exposure and worsened to a semi-coma state at 26 days after onset. When he started rehabilitation six weeks after the CO exposure, he had impaired consciousness, with a Glasgow Coma Scale score of 8 and a Coma Recovery Scale-Revised score of 8. On 6-week DTT, decreased neural connectivity of the upper ARAS between the intralaminar thalamic nucleus and the cerebral cortex was observed in both frontal cortices, basal forebrains, basal ganglia and thalami. The lower dorsal ARAS was not reconstructed on the right side, and was thin on the left side. The lower ventral ARAS was not reconstructed on either side.
Using DTT, we demonstrated injury of the ARAS in a patient with impaired consciousness following DPHL. Our result suggests that injury of the ARAS is a plausible pathogenetic mechanism of impaired consciousness in patients with DPHL.
KeywordsDelayed post-hypoxic leukoencephalopathy Hypoxic brain injury Ascending reticular activating system Consciousness Diffusion tensor tractography
Delayed post-hypoxic leukoencephalopathy (DPHL), a rare clinical condition, is a demyelinating syndrome characterized by neurological relapse after an initial recovery from hypoxic brain injury caused by carbon monoxide (CO) poisoning, overdose of drug, and myocardial infarction [1–3]. The majority of DPHL cases are associated with CO poisoning . Lee and Marsden divided DPHL into two general clinical categories: parkinsonism (masked face, rigidity, tremor, dystonic posturing, agitation) and akinetic mutism (apathetic and developed functional bowel and minimal primitive responses to pain) [5–13]. However, very little is known about impaired consciousness following DPHL.
Hypoxic brain injury predominantly involves the gray matter. MRI is recognized as the most sensitive and common imaging tool for hypoxic brain injury . In contrast, DPHL predominantly involves the white matter. Many studies have reported abnormality of the white matter including basal ganglia following DPHL using neuroimaging tools such as conventional MRI, diffusion weight imaging, and MR spectroscopy [5–13].
Recently developed diffusion tensor tractography (DTT), derived from diffusion tensor imaging (DTI), has the unique capability to estimate the neural tract in the white matter and is able to find the subtle or invisible neural injury by detection of characteristics of water diffusion . Injury of the ascending reticular activating system (ARAS), which is responsible for consciousness, has been reported in patients with hypoxic brain injury [16, 17]. However, no study of injury of the ARAS in patients with DPHL has been reported.
In this study, using DTT, we report on a patient with impaired consciousness concurrent with injury of the ARAS following DPHL.
Magnetic resonance imaging and diffusion tensor imaging
Imaging parameters for T2-weighted MRI were as follows: acquisition matrix = 265 × 224, field of view = 210 × 210 mm2, repetition time = 4224.1 ms, echo time = 100 ms, number of excitations = 2, and a slice thickness of 5 mm with a gap of 2.2 mm. DTI data were acquired at six weeks after onset using a six-channel head coil on a 1.5 T Philips Gyroscan Intera with single-shot echo-planar imaging. Imaging parameters were as follows: acquisition matrix = 96 × 96; reconstructed to matrix = 192 × 192; field of view = 240 × 240 mm2; repetition time = 10,398 ms; echo time = 72 ms; echo-planar imaging factor = 59; b = 1000s/mm2; and a slice thickness = 2.5 mm. Affine multi-scale two-dimensional registration at the Oxford Centre for Functional Magnetic Resonance Imaging of Brain (FMRIB) Software Library was used to correct head motion effect and image distortion. Fiber tracking used FMRIB Diffusion (5000 streamline samples, 0.5 mm step lengths, curvature thresholds = 0.2), a probabilistic tractography method . Three portions of the ARAS were reconstructed by selection of fibers passing through region of interest (ROI) as follows [21–23]: the upper ARAS, in which the neural connectivity of the intralaminar thalamic nucleus (ILN, ROI 1) to the cerebral cortex was analyzed, the dorsal lower ARAS, between the pontine reticular formation (RF, ROI 1) and the ILN (ROI 2), and the ventral lower ARAS, between the pontine RF (ROI 1) and the hypothalamus (ROI 2). Out of 5000 samples generated from the seed voxel, results for fiber tracking were applied at a threshold of two streamlines for the dorsal and ventral lower ARAS and 10 streamlines for the upper ARAS.
On 6-week DTT, decreased neural connectivity of the upper ARAS between the ILN and the cerebral cortex was observed in both frontal cortices, basal forebrains, basal ganglia and thalami (Fig. 1). The dorsal lower ARAS between the pontine RF and the ILN was not reconstructed on the right side and thin on the left side. The ventral lower ARAS between the pontine RF and the hypothalamus was not reconstructed on either side.
Discussion and conclusions
In the current study, three portions of the ARAS (the dorsal lower ARAS, ventral lower ARAS and upper ARAS) in a patient with impaired consciousness following DPHL caused by CO poisoning were evaluated using DTT. We found that these three portions of the ARAS were injured in both hemispheres: the upper ARAS – decreased neural connectivity to both frontal cortexes, basal forebrains, basal ganglia and thalami, the dorsal lower ARAS – non-reconstruction in the right side and narrowing in the left side and the ventral lower ARAS –non-reconstruction in both sides. We believe that the impaired consciousness in this patient was ascribed to the injury of the three portions of the ARAS.
Many studies have reported abnormality of the white matter including basal ganglia (caudate nucleus, putamen, and globus pallidus) in patients with DPHL using various neuroimaging tools including conventional MRI [5–13]. Neurological manifestations were observed as follows: 1) cognitive impairments - confusion, disorientation, executive dysfunction, attention deficit, and akinetic mutism 2) motor symptoms - spasticity, hyper-reflexia, bradykinesia, rigidity, tremor, gait disturbance, dystonia 3) hallucinations, and 4) dysautonomia [5–13]. Regarding DTI, as far as we are aware, only one study was reported on patients with DPHL . In 2008, Kenshi et al. demonstrated extensive white matter injury using DTI parameters (fractional anisotropy and mean diffusivity) in two patients with carbon monoxide intoxication (patient 1: frontal and parietal regions, globus pallidus, and corpus callosum and patient 2: globus pallidus) and showed neurological manifestations as follows: 1) patient 1 - akinetic mutism, disorientation, gait disturbance and 2) patient 2 - akinetic mutism . To the best of our knowledge, this is the first DTT study to demonstrate injury of the ARAS in a patient with DPHL.
In conclusion, using DTT, we demonstrated injury of the ARAS in a patient with impaired consciousness following DPHL. Our result suggests injury of the ARAS is a plausible pathogenetic mechanism of impaired consciousness in patients with DPHL. However, because it is a single case report, this study is limited. In addition, several limitations of this study should be considered. First, use of DTT could lead to both false positive and negative results due to multiple fiber orientations in a voxel . Second, we could not provide correlation between cognitive function and DTI anatomical site. Third, based on the blood test, we could not completely ruled out whether it affected neurogical status of the patient. Therefore, we suggest that further studies including large numbers of patients and overcoming limitations of this study should be encouraged.
Analysis and interpretation of data in writing the manuscript was supported by the Medical Research Center Program (2015R1A5A2009124) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning.
Availability of data and materials
All data underlying this article will be provided by the corresponding author (Hyeok Gyu Kwon) upon request.
SHJ participated the design of this study and collected the clinical raw data, writing the manuscript. HGJ carried out analysis of data, writing the manuscript. Both authors read and approved the final manuscript.
Ethics approval and consent to participate
The patient’s wife understood the purpose of the study and provided written, informed consent prior to participation. The study protocol was approved by the Institutional Review Board of a Yeungnam university hospital.
Consent for publication
Written informed consent was obtained from the patient’s wife for publication of this Case Report and any accompanying images. A copy of the written consent is available for review by the Editor of this journal.
The authors declare that they have no competing interests.
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- Plum F, Posner JB, Hain RF. Delayed neurological deterioration after anoxia. Arch Intern Med. 1962;110:18–25.View ArticlePubMedGoogle Scholar
- Shprecher D, Mehta L. The syndrome of delayed post-hypoxic leukoencephalopathy. NeuroRehabilitation. 2010;26(1):65–72.PubMedPubMed CentralGoogle Scholar
- Zamora CA, Nauen D, Hynecek R, Ilica AT, Izbudak I, Sair HI, Gujar SK, Pillai JJ. Delayed posthypoxic leukoencephalopathy: a case series and review of the literature. Brain Behav. 2015; 5(8):e00364. doi:https://doi.org/10.1002/brb3.364.
- Choi IS. Delayed neurologic sequelae in carbon monoxide intoxication. Arch Neurol. 1983;40(7):433–5.View ArticlePubMedGoogle Scholar
- Lee MS, Marsden CD. Neurological sequelae following carbon monoxide poisoning clinical course and outcome according to the clinical types and brain computed tomography scan findings. Mov Disord. 1994;9(5):550–8. doi:https://doi.org/10.1002/mds.870090508.View ArticlePubMedGoogle Scholar
- Barnett MH, Miller LA, Reddel SW, Davies L. Reversible delayed leukoencephalopathy following intravenous heroin overdose. J Clin Neurosci. 2001;8(2):165–7. doi:https://doi.org/10.1054/jocn.2000.0769.View ArticlePubMedGoogle Scholar
- Lam SP, Fong SY, Kwok A, Wong T, Wing YK. Delayed neuropsychiatric impairment after carbon monoxide poisoning from burning charcoal. Hong Kong Med J. 2004;10(6):428–31.PubMedGoogle Scholar
- Hsiao CL, Kuo HC, Huang CC. Delayed encephalopathy after carbon monoxide intoxication--long-term prognosis and correlation of clinical manifestations and neuroimages. Acta Neurol Taiwanica. 2004;13(2):64–70.Google Scholar
- Shprecher DR, Flanigan KM, Smith AG, Smith SM, Schenkenberg T, Steffens J. Clinical and diagnostic features of delayed hypoxic leukoencephalopathy. J Neuropsychiatry Clin Neurosci. 2008;20(4):473–7. doi:10.1176/appi.neuropsych.20.4.473. 10.1176/jnp.2008.20.4.473.View ArticlePubMedGoogle Scholar
- Rozen TD. Rapid resolution of akinetic mutism in delayed post-hypoxic leukoencephalopathy with intravenous magnesium sulfate. NeuroRehabilitation. 2012;30(4):329–32. doi:https://doi.org/10.3233/NRE-2012-0763.PubMedGoogle Scholar
- Meyer MA. Delayed post-hypoxic leukoencephalopathy: case report with a review of disease pathophysiology. Neurol Int. 2013;5(3):e13. doi:https://doi.org/10.4081/ni.2013.e13.
- Huisa BN, Gasparovic C, Taheri S, Prestopnik JL, Rosenberg GA. Imaging of subacute blood-brain barrier disruption after methadone overdose. J Neuroimaging. 2013;23(3):441–4. doi:https://doi.org/10.1111/j.1552-6569.2011.00669.x.View ArticlePubMedGoogle Scholar
- Geraldo AF, Silva C, Neutel D, Neto LL, Albuquerque L. Delayed leukoencephalopathy after acute carbon monoxide intoxication. J Radiol Case Rep. 2014;8(5):1–8. doi:https://doi.org/10.3941/jrcr.v8i5.1721.PubMedPubMed CentralGoogle Scholar
- Huang BY, Castillo M. Hypoxic-ischemic brain injury: imaging findings from birth to adulthood. Radiographics. 2008; 28(2):417–439; quiz 617. doi:https://doi.org/10.1148/rg.282075066.
- Behrens TE, Berg HJ, Jbabdi S, Rushworth MF, Woolrich MW. Probabilistic diffusion tractography with multiple fibre orientations: What can we gain? Neuroimage. 2007;34(1):144–55. doi:https://doi.org/10.1016/j.neuroimage.2006.09.018.View ArticlePubMedGoogle Scholar
- Jang SH, Kim SH, Lim HW, Yeo SS. Injury of the lower ascending reticular activating system in patients with hypoxic-ischemic brain injury: diffusion tensor imaging study. Neuroradiology. 2014;56(11):965–70. doi:https://doi.org/10.1007/s00234-014-1419-y.View ArticlePubMedGoogle Scholar
- Jang SH, Hyun YJ, Lee HD. Recovery of consciousness and an injured ascending reticular activating system in a patient who survived cardiac arrest: A case report. Medicine (Baltimore). 2016;95(26):e4041. doi:https://doi.org/10.1097/MD.0000000000004041.
- Teasdale G, Jennett B. Assessment of coma and impaired consciousness. A practical scale. Lancet. 1974;2(7872):81–4.View ArticlePubMedGoogle Scholar
- Giacino JT, Kalmar K, Whyte J. The JFK coma recovery scale-revised: measurement characteristics and diagnostic utility. Arch Phys Med Rehabil. 2004;85(12):2020–9.View ArticlePubMedGoogle Scholar
- Smith SM, Jenkinson M, Woolrich MW, Beckmann CF, Behrens TE, Johansen-Berg H, Bannister PR, De Luca M, Drobnjak I, Flitney DE, et al. Advances in functional and structural MR image analysis and implementation as FSL. Neuroimage. 2004;23(Suppl 1):S208–19. doi:https://doi.org/10.1016/j.neuroimage.2004.07.051.
- Yeo SS, Chang PH, Jang SH. The ascending reticular activating system from pontine reticular formation to the thalamus in the human brain. Front Hum Neurosci. 2013; 7:416. doi:https://doi.org/10.3389/fnhum.2013.00416.
- Jang SH, Lim HW, Yeo SS. The neural connectivity of the intralaminar thalamic nuclei in the human brain: a diffusion tensor tractography study. Neurosci Lett. 2014;579:140–4. doi:https://doi.org/10.1016/j.neulet.2014.07.024.View ArticlePubMedGoogle Scholar
- Jang SH, Kwon HG. The ascending reticular activating system from pontine reticular formation to the hypothalamus in the human brain: a diffusion tensor imaging study. Neurosci Lett. 2015;590:58–61. doi:https://doi.org/10.1016/j.neulet.2015.01.071.View ArticlePubMedGoogle Scholar
- Terajima K, Igarashi H, Hirose M, Matsuzawa H, Nishizawa M, Nakada T. Serial assessments of delayed encephalopathy after carbon monoxide poisoning using magnetic resonance spectroscopy and diffusion tensor imaging on 3.0T system. Eur Neurol. 2008;59(1–2):55–61. doi:https://doi.org/10.1159/000109262.
- Yamada K. Diffusion tensor tractography should be used with caution. Proc Natl Acad Sci U S A. 2009; 106(7):E14; author reply E5. doi:https://doi.org/10.1073/pnas.0812352106.