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Neuroserpin polymorphisms and stroke risk in a biracial population: the stroke prevention in young women study
© Cole et al; licensee BioMed Central Ltd. 2007
Received: 11 April 2007
Accepted: 25 October 2007
Published: 25 October 2007
Neuroserpin, primarily localized to CNS neurons, inhibits the adverse effects of tissue-type plasminogen activator (tPA) on the neurovascular unit and has neuroprotective effects in animal models of ischemic stroke. We sought to evaluate the association of neuroserpin polymorphisms with risk for ischemic stroke among young women.
A population-based case-control study of stroke among women aged 15–49 identified 224 cases of first ischemic stroke (47.3% African-American) and 211 age-matched control subjects (43.1% African-American). Neuroserpin single nucleotide polymorphisms (SNPs) chosen through HapMap were genotyped in the study population and assessed for association with stroke.
Of the five SNPs analyzed, the A allele (frequency; Caucasian = 0.56, African-American = 0.42) of SNP rs6797312 located in intron 1 was associated with stroke in an age-adjusted dominant model (AA and AT vs. TT) among Caucasians (OR = 2.05, p = 0.023) but not African-Americans (OR = 0.71, p = 0.387). Models adjusting for other risk factors strengthened the association. Race-specific haplotype analyses, inclusive of SNP rs6797312, again demonstrated significant associations with stroke among Caucasians only.
This study provides the first evidence that neuroserpin is associated with early-onset ischemic stroke among Caucasian women.
A growing body of evidence indicates that the interaction between tissue-type plasminogen activator (tPA) and neuroserpin regulates not only the permeability of the neurovascular unit but also the fate of the ischemic tissue in response to the ischemic insult [2–4, 11, 12]. Indeed, following experimental middle cerebral artery occlusion (MCAO) there is a rapid increase in tPA activity and neuroserpin expression in the astrocyte-endothelial cell interface [2, 4]. Either genetic deficiency of tPA or its inhibition with neuroserpin results in a significant decrease not only in the volume of the ischemic lesion but also in the permeability of the blood-brain barrier . Furthermore, it has been demonstrated that treatment with neuroserpin following ischemic stroke, or overexpression of the neuroserpin gene, results in a significant decrease in the volume of the ischemic area as well as in the number of apoptotic cells [2, 3]. Thus, it is highly plausible that endogenous neuroserpin may influence whether or not transient ischemia results in an ischemic stroke.
These findings led us to hypothesize that specific variants in the neuroserpin gene may provide improved or diminished protection with respect to the risk of ischemic stroke. To test this hypothesis, we genotyped several common neuroserpin single nucleotide polymorphisms (SNPs) and tested genotypic and haplotypic association with stroke in a previously collected case-control sample of ischemic stroke among young women.
The Stroke Prevention in Young Women Study 2 (SPYW2) is a population-based case-control study that was designed to examine genetic risk factors for ischemic stroke in young women. The term "population-based" indicates that the cases and their comparison group are representative of the general population and were identified from the same defined population, which included all of Maryland (except the far Western panhandle), Washington DC, and the southern portions of both Pennsylvania and Delaware. Cases included 239 female patients 15 to 49 years of age with a first cerebral infarction as identified by discharge diagnosis at 52 regional hospitals and through direct referral by regional neurologists. The methods for discharge surveillance, chart abstraction, and case adjudication have been described previously [13–15]. The adjudication of stroke cases was performed blinded to genetic information. Stroke cases were classified as having a probable, possible or undetermined etiology as per prior description [13, 14]. We excluded fifteen subjects from our analyses based on a modification of the exclusion criteria used in the Siblings With Ischemic Stroke Study (SWISS)  protocol: sickle cell disease (1), CNS vasculitis by angiogram and clinical criteria (3), post-radiation arteriopathy (1), endocarditis (3), neurosyphillis (1), mechanical prosthetic heart valves (2), left atrial myxoma (1), and cocaine use in the 48 hours prior to their stroke (3). Control subjects were 212 women without a history of stroke, identified by random-digit-dialing and were frequency matched to the cases by age and geographic region of residence. One control was excluded from analyses based on a history of sickle cell disease. Thus, the sample for genetic analyses consisted of 224 cases and 211 controls.
Cases and controls were grouped into the following race and ethnic categories: Caucasian (non-Hispanic) (n = 95 cases and 99 controls), African-American (n = 105 cases and 91 controls), and other (including Hispanic, Asian, American-Indian, etc.) (n = 24 cases and 21 controls). Because of the small size and heterogeneity of the latter group, it was not analyzed separately, but was included in analyses on the combined total study group (n = 224 cases and 211 controls). Haplotypic association analyses were conducted on only the Caucasian (non-Hispanic) and the African-American groups. Strokes were further classified by subtype; the atherosclerotic group included 27 cases with either probable or possible atherosclerotic mechanism; the cardiac group included 14 cases with a probable cardiac source of embolism; the probable dissection group included 13 cases confirmed by neuroimaging; the lacunar group included 45 cases of symptomatic small deep lesions on neuroimaging studies or classic lacunar syndromes regardless of other potential causes; and the probable hematologic group included 9 cases. These categories were not mutually exclusive. There were 125 non-lacunar stroke cases of undetermined etiology.
Ethical approval for the study was obtained from the University of Maryland School of Medicine Institutional Review Board.
In an effort to eliminate redundancy in the information provided by the SNPs genotyped, a set of haplotype tagging SNPs were identified through HapMap  for both African-Americans and Caucasians. To represent our Caucasian population we identified SNPs that capture 80% of haplotype diversity among European Caucasians. These SNPs were then "force included" to determine the additional tagging SNPs needed fromYorubans to capture 80% of haplotype diversityin our African-American population. A minor allele frequency of >.05 was required. After removing markers with minor allele frequency <0.05, we prioritized the remaining markers by: 1) non-redundant haplotype tagging coverage among both races, 2) distribution throughout the gene, and 3) maximizing the allele frequencies for both SNP alleles. On this basis six SNPs were genotyped in our stroke cohort, including: rs2420034, rs6797312, rs6775296, rs2055028, rs1027242, and rs13090569. Complete HapMap coverage was not attained.
Genotyping methods for the case/control population
Genotyping was conducted using DNA isolated from whole blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Valencia, CA). SNP genotyping was performed by the Taqman method (Applied Biosystems, Foster City, CA). This method is based on four primers, two flanking the SNP that are used to amplify the DNA surrounding the SNP and two that were labeled with different fluorescent dyes, one for each alternative allele. The original form of the labeled primer has a quencher in close proximity to the dye. When the 5'→3' exonuclease activity of DNA polymerase disrupts the primer hybridized to the single strand DNA during the PCR, the quencher and dye are released, producing a measurable level of fluorescence. The reaction protocol was specified in manufacturer's instructions included with each individual primer set.
All statistical analyses were performed using SAS®, Version 9.1 (SAS Institute, Cary, NC). We compared means by two-sided t-tests and proportions by χ2 tests. For those SNPs that satisfied Hardy-Weinberg equilibrium, additive, dominant and recessive models were used to test the effect of genotype on stroke risk. Analyses were conducted in the total population, and also in the African-American and Caucasian women separately. Single SNP association analyses were not adjusted for multiple comparisons because so few SNPs were evaluated and our study was considered to be hypothesis-generating.
Adjusted odds ratios from logistic regression were used to determine whether the presence of the risk allele was associated with an increased risk for stroke after controlling for potential confounders. The minimally adjusted model included age and race. The environmental model included age, race, current cigarette smoking, and oral contraceptive pills (OCP) use. In addition to the covariates of the environmental model, the environmental/vascular risk factor model was adjusted for hypertension, diabetes mellitus, and history of angina or myocardial infarction (angina/MI). Age, race, current cigarette smoking status, and OCP use were based on self-report by subjects (or proxies, if a participant was unable to answer). Hypertension and diabetes mellitus were determined by asking study participants (or a proxy) if a physician had ever told them they had the condition.
Within each ethnic group, analyses were stratified by standard risk factors (age, OCP use, current cigarette smoking, hypertension, diabetes mellitus, and history of angina or myocardial infarction) and stroke subtype (atherosclerotic, cardiac, dissection, lacunar, hematologic, and stroke of undetermined etiology (all other stroke)).
Patterns of linkage disequilibrium (LD) were determined for each race group (African-Americans and Caucasians) using pairwise estimates of D' as implemented in Haploview . Race-specific haplotypic association analyses were performed using Haplo.stats  in the statistical software package . Individual haplotypes were estimated in the HaploStats package using the haplo.em program, which infers haplotypes and makes haplotype assignments using a progressive insertion algorithm based on an Expectation-Maximization approach, as implemented in the "SNPHAP" software package . Haplotypes first underwent race-specific non-adjusted analyses to evaluate for association with stroke using the haplo.score program. Consistent with the SNP analyses, significant haplotypes were analyzed using age-adjusted, environmental and environmental/vascular models, as well as analyses stratified by stroke subtype using the haplo.glm program. Empirical p-values were estimated for haplotypic association tests in haplo.score using a simulation-based approach, thus correcting for multiple comparisons.
Characteristics by case-control status.
Cases (N = 224)
Controls (N = 211)
Mean age (years)
Diabetes mellitus (%)
Current smokers (%)
SNP association analyses
SNPs analyzed in complete case-control study population including allelic variants, position, gene region, genotype call rate, and minor allele frequencies as stratified by race and case/control status.
SNP rs number – Alleles
Position* – Region
rs2420034 – A/T
168937699 – Intron 1
rs6797312 – A/T
168969997 – Intron 1
rs6775296 – A/G
168973594 – Intron 1
rs2055028 – A/G
169013200 – Intron 6
rs1027242 – C/T
SNPs associated with stroke
SNP rs6797312 – Stratified by race in dominant model. Age-adjusted, environmental, and environmental/vascular models demonstrating adjusted odds ratios, 95% confidence intervals, and p-values
Environmental Model *
Environmental/Vascular Model **
Among Caucasians, the 3 SNP haplotype rs24240034(A)-rs6797312(A)-rs6775296(A) (haplotype frequency = 25%) was associated with stroke (p = 0.021) in an unadjusted model when evaluating all strokes combined. These associations persisted after controlling for other vascular risk factors in age-adjusted (p = 0.019), environmental (p = 0.004), and environmental/vascular (p = 0.015) models respectively. The stroke of undetermined etiology subgroup demonstrated a trend toward association when analyzed in the unadjusted model (p = 0.10), age-adjusted (p = 0.10), environmental (p = 0.032), and environmental/vascular (p = 0.032) models respectively. Neither of the 2 SNP haplotypes demonstrated associations with stroke.
Among African-Americans, no rs6797312–rs6775296 haplotype demonstrated an association.
Although several studies have demonstrated that neuroserpin plays a role in cerebral ischemia [2–4, 22], our study is the first to demonstrate that specific neuroserpin SNPs and haplotypes are associated with the risk of ischemic stroke. While the primary function of neuroserpin appears to be inhibition of tPA in both diseased and non-diseased states , increasing evidence indicates that the tPA-neuroserpin axis plays a major role in the regulation of neurovascular unit permeability during cerebral ischemia [2–4, 22]. Hence, the beneficial or detrimental role of tPA in cerebral ischemia is location dependent, with intravascular tPA promoting fibrinolysis, and extravascular tPA promoting blood-brain barrier disruption and/or neurotoxicity as regulated by neuroserpin. Furthermore, animal studies of induced ischemic brain injury have demonstrated that the administration of exogenous neuroserpin decreases these effects, hence playing a neuroprotective role, as evidenced by smaller stroke volumes . Thus, it is logical to hypothesize that specific variants in the neuroserpin gene may influence whether or not transient ischemia results in ischemic stroke.
Our study indicates that a specific neuroserpin SNP (rs6797312), and haplotypes including this SNP, are associated with ischemic stroke risk in Caucasian women. The haplotype associations seen in the race-stratified all ischemic stroke groups and the stroke of undermined etiology subtype, suggest that neuroserpin variants may offer varying detrimental or protective effects on ischemia that may be global in nature. Therefore, one could cautiously interpret our results to indicate that SNP rs6797312, or more likely that a nearby SNP in linkage disequilibrium with SNP rs6797312, acts to tilt the tPA-neuroserpin axis towards a less favorable state regarding stroke risk or neuroprotection.
Our study has several limitations. First, our study does not provide any mechanistic information regarding stroke risk or protection. Specifically, we did not determine if our SNPs were associated with increased or decreased neuroserpin activity. Secondly, our study population is relatively small and could be underpowered to detect modest effects, particularly in the stroke subtype analyses. Additionally, even though we evaluated a small set of tagging SNPs identified using LD patterns in HapMap data; the tagging SNPs did not capture all variation in the gene among both race subgroups. Lastly, although we performed numerous analyses, no correction was made for multiple comparisons among the single SNP association analyses, allowing for the possibility that our results could be attained through chance alone. However, the estimation of empirical p-values for haplotypic association tests involving SNP rs6797312 accounts for the issue of multiple comparisons, making a false positive association in haplotype analyses less likely.
This study provides the first evidence that neuroserpin is associated with early-onset ischemic stroke among Caucasian women.
We are indebted to the following members of the Stroke Prevention in Young Women research team for their dedication: Esther Berrent, Kathleen Caubo, Julia Clark, Mark Dobbins, Mohammed Huq, Ann Maher, Tamar Pair, Mary Simmons, Mary J. Sparks, Mark Waring, Latasha Williams, and Nancy Zappala.
The authors would like to acknowledge the assistance of the following individuals who have sponsored the Stroke Prevention in Young Women Study at their institution: Clifford Andrew, MD; Merrill Ansher, Brian Avin, MD; MD; Harjit Bajaj, MD; Robert Baumann, MD; Nicholas Buendia, MD, Young Ja Cho, MD; Kevin Crutchfield, MD; Terry Detrich, MD; Mohammed Dughly, MD; Boyd Dwyer, MD; Jerold Fleishman, MD; Stuart Goodman, MD, PhD; Adrian Goldszmidt, MD; Kalpana Hari Hall, MD; Walid Kamsheh, MD; John Kelly, MD; Harry Kerasidis, MD; Mehrullah Khan, MD; Ramesh Khurana, MD; Ruediger Kratz MD; Somchai Laowattana, MD; William Leahy, MD; Alan Levitt, MD; Bruce Lobar, MD; Paul Melnick, MD; Harshad Mody, MD; Seth Morgan, MD; Howard Moses, MD; Francis Mwaisela, MD; Sivarama Nandipati, MD; Maciej Poltorak, MD; Thaddeus Pula, MD; Phillip Pulaski, MD; Neelupali Reddy, MD; Perry Richardson, MD; Solomon Robbins, MD; Michael Sellman, MD, PhD; Barney Stern, MD; Jack Syme, MD; Richard Taylor, MD; Dean Tippett, MD; Michael Weinrich, MD; Roger Weir, MD; Richard Weisman, MD; Laurence Whicker, MD; Robert Wityk, MD; James Yan, MD and Manuel Yepes, MD.
In addition, the study could not have been completed without the support from the administration and medical records staff at the following institutions: In Maryland: Anne Arundel Medical Center, Bon Secours Hospital, Calvert Memorial Hospital, Carroll County General Hospital, Chester River Hospital, Civista Medical Center, Department of Veterans Affairs Medical Center in Baltimore, Doctors Community Hospital, Dorchester Hospital, Franklin Square Hospital Center, Frederick Memorial Hospital, Good Samaritan Hospital, Greater Baltimore Medical Center, Harbor Hospital Center, Hartford Memorial Hospital, Holy Cross Hospital, Howard County General Hospital, Johns Hopkins Bayview, The Johns Hopkins Hospital, Kernan Hospital, Laurel Regional Hospital, Maryland General Hospital, McCready Memorial Hospital, Memorial Hospital at Easton, Mercy Medical Center, Montgomery General Hospital, North Arundel Hospital, Northwest Hospital Center, Peninsula Regional Medical Center, Prince George's Hospital Center, Saint Agnes Hospital, Saint Joseph Medical Center, Saint Mary's Hospital, Shady Grove Adventist Hospital, Sinai Hospital of Baltimore, Southern Maryland Hospital Center, Suburban Hospital, Union Hospital Cecil County, The Union Memorial Hospital, University of Maryland Medical System, Upper Chesapeake Medical Center, Washington Adventist Hospital and Washington County Hospital; in Washington DC: The George Washington University Medical Center; Georgetown University Hospital; Hadley Memorial Hospital; Howard University Hospital; National Rehabilitation Hospital; Providence Hospital; Sibley Memorial Hospital; and the Washington Hospital Center; in Pennsylvania: Gettysburg Hospital.
Dr. Cole's efforts on this project were supported in part by the Department of Veterans Affairs, Baltimore, Office of Research and Development, Medical Research Service; the Department of Veterans Affairs Stroke Research Enhancement Award Program; the University of Maryland General Clinical Research Center (Grant M01 RR 165001), General Clinical Research Centers Program, National Center for Research Resources, NIH, and; an American Heart Association Beginning Grant-in-Aid (Grant 0665352U). Dr. Kittner was supported in part by the Department of Veterans Affairs, Baltimore, Office of Research and Development, Medical Research Service, and Geriatrics Research, Education and Clinical Center, and Stroke Research Enhancement Award Program; a Cooperative Agreement with the Division of Adult and Community Health, Centers for Disease Control and Prevention; the National Institute of Neurological Disorders and Stroke and the NIH Office of Research on Women's Health; the National Institute on Aging Pepper Center (Grant P60 12583); and the University of Maryland General Clinical Research Center (Grant M01 RR 165001), General Clinical Research Centers Program, National Center for Research Resources, NIH. Dr. Sorkin was supported by the Baltimore VA Medical Center, Office of Research and Development, Medical Research Service, and Geriatrics Research, Education, and Clinical Center; the University of Maryland Claude D. Pepper Older Americans Independence Center; the Clinical Nutrition Research Unit of the University of Maryland, and; the Baltimore VA Medical Center, Center for Excellence in Robotics.
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