Research article | Open | Open Peer Review | Published:
Latent profile analysis in frontotemporal lobar degeneration and related disorders: clinical presentation and SPECT functional correlates
BMC Neurologyvolume 7, Article number: 9 (2007)
Frontotemporal Lobar Degeneration (FTLD) thus recently renamed, refers to a spectrum of heterogeneous conditions. This same heterogeneity of presentation represents the major methodological limit for the correct evaluation of clinical designation and brain functional correlates. At present, no study has investigated clinical clusters due to specific cognitive and behavioural disturbances beyond current clinical criteria.
The aim of this study was to identify clinical FTLD presentation, based on cognitive and behavioural profile, and to define their SPECT functional correlations.
Ninety-seven FTLD patients entered the study. A clinical evaluation and standardised assessment were preformed, as well as a brain SPECT perfusion imaging study. Latent Profile Analysis on clinical, neuropsychological, and behavioural data was performed. Voxel-basis analysis of SPECT data was computed.
Three specific clusters were identified and named "pseudomanic behaviour" (LC1), "cognitive" (LC2), and "pseudodepressed behaviour" (LC3) endophenotypes. These endophenotypes showed a comparable hypoperfusion in left temporal lobe, but a specific pattern involving: medial and orbitobasal frontal cortex in LC1, subcortical brain region in LC2, and right dorsolateral frontal cortex and insula in LC3.
These findings provide evidence that specific functional-cluster symptom relationship can be delineated in FTLD patients by a standardised assessment. The understanding of the different functional correlates of clinical presentations will hopefully lead to the possibility of individuating diagnostic and treatment algorithms.
Frontotemporal Lobar Degeneration (FTLD) refers to a spectrum of heterogeneous conditions . The disease designation and the disease concept were renamed in the past few years as different clinical criteria have been evolved over time. Until now, all these syndromes have been recognised to present with cognitive and language disturbances, personality changes and behavioural symptoms, including different clinical and anatomical conditions. Primary Progressive Aphasia (PPA), also called progressive nonfluent aphasia (PNFA), typically occurs with dominant frontotemporal atrophy [2, 3]. These patients do not initially demonstrate behavioural abnormality. If the dominant temporal lobe is involved, FTLD presents with anomia, and the result is often impairment in language comprehension and loss of meaning, called Semantic Dementia (SD), which is often associated with behavioural abnormalities . When behavioural abnormalities are the presenting feature, usually with nondominant temporal and frontal atrophy, the label "behavioural variant" (bvFTD) is used . The recognition of a genetic and clinical overlap of FTLD with Corticobasal Degeneration (CBD) and Progressive Supranuclear Palsy (PSP) suggested the inclusion of these relatively rare conditions under the same nosographic label of FTLD [5, 6].
Therefore, different presentations were separated into independent entities, creating a terminological proliferation and multiplying rare diseases. The classification of these diverse conditions remains controversial, some groups perceive FTLD as a unitary continuum whereas others describe more discrete syndromes [5, 7].
Pathological fractionation did not lag behind, as histochemistry and molecular biology yielded new features . Because of excessive deposits of hyperphosphorylated tau in affected neurons and glia, the underlying pathology of FTLD was regarded as a tauopathy. Paradoxically, the general label of tauopathy has been expanded to include cases in which tau was not found in the brain, and the vast majority of FTLD pathology is actually recognized by ubiquitin positive and tau negative inclusions.
FTLD conditions, however, have more in common than previously recognised, and the term Pick's Complex was chosen to signify their cohesion .
In fact, an integrative approach based on clinicopathological correlation suggests that not only the bvFTD, SD, and PNFA overlap clinically and pathologically, but the extrapyramidal component, i.e. CBD and PSP, should be considered part of an overall entity named FTLD [1, 5]. All these syndromes were recognised to overlap throughout disease progression, and it was recently demonstrated that there is a convergence of the syndromes in the course of the disease .
Brain imaging techniques have been successfully used to characterise the different syndromes, allowing us to explore the involved neural systems, and helping us in comprehend their pathogenetic mechanisms [6, 10, 11]. The question that remains opens, however, is whether a single neural network underscoring FTLD or different entities might be discriminated by structural or functional neuroimaging. The further problem in evaluating FTLD patients is that, in most cases, more than one behavioural or cognitive symptom occurs in the same patient . In addition, the use pre-defined clinical criteria aimed to verify such imaging data may imply a risk of circularity.
At present, clinical clusters might provide the opportunity to identify specific cognitive and behavioural patterns beyond current clinical criteria.
With this aim in mind, we applied Latent Profile Analysis (LPA), a latent variable model that is used to detect subgroups or syndromes based on the observed relationship between chosen continuous indicators or symptoms . LPA is here employed to rationalise the large clinical and neuropsychological data set with an open approach, allowing us to reveal discrete clinical presentation.
Once the data-driven classification has been formed, its coherence was verified by functional neuroimaging information.
This study is part of an ongoing research program aimed at evaluating the core feature of FTLD. Patients who met the Neary  and McKhann  criteria for FTLD were consecutively recruited from March 2003 through July 2005 from the "Center for Aging Brain and Neurodegenerative Diseases", University of Brescia, Italy.
All subjects underwent a somatic and neurological evaluation, and routine laboratory examination, a brain structural Magnetic Resonance Imaging (MRI) study, and a brain functional Single Photon Emission Tomography (SPECT) study.
The diagnostic assessment involved a review of full medical history, a semi-structured neurological examination, and a complete mental status evaluation by two independent and experienced reviewers (B.B., A.P.). Only patients with full consensus agreement by the reviewers were enrolled.
Motor impairment was evaluated using the motor subscale of Unified Parkinson Disease Rating Scale (UPDRS) III . Global cognitive function assessment was made according to a standardized battery, including the Mini-Mental State Examination (MMSE) . The neuropsychological assessment was made with the following tests: Raven Colored Progressive Matrices , Controlled Oral Word Association Test and Category Fluency , Clock Drawing Test , Rey Complex Figure Copy and Recall , Story Recall Test , Digit Span , Trail Making Test A and B , Token Test , and De Renzi Imitation Test . Instrumental Activities of Daily Living (IADL) , and Basic Activities of Daily Living (BADL)  were assessed as well. Behavioural and psychiatric disturbances were evaluated by Neuropsychiatry Inventory (NPI) , and Frontal Behavioual Inventory (FBI) .
Patients considered to have a positive family history were those who had a first-degree relative with dementia, Parkinsonism, or motor neuron disease. No patients belonging to the same family were included.
All participants were made fully aware of the research goals, and the signature of an informed consent was required from all subjects. The work was conducted in accordance with local clinical research regulations and in conformity with the Helsinki Declaration.
The Neary  and McKhann criteria  for FTLD were fulfilled by all subjects. In particular, bvFTD was defined by character change and disorder of social conduct. SD was defined by a prominent comprehension disorder (impaired understanding of word meaning and/or object identity), difficulty in naming, and asking for the name of nouns and objects. PNFA was diagnosed when the first symptom was an isolated disorder of expressive language when other aspects of cognition and daily living functions were relatively well preserved. CBD exhibited unilateral rigidity and apraxia, some of them having 'alien hand'. In these patients the extrapyramidal syndrome developed first and was followed by cognitive changes. PSP showed vertical gaze palsy, repetitive falls, axial rigidity and pseudobulbar palsy; a poor response to L-dopa treatment was counted as an additional inclusion criterion.
In FTLD patients, an adjunctive inclusion criterion was an initial diagnosis confirmation after the patient had been followed periodically for a 1.5 year period.
Stringent exclusion criteria were applied as follows: a) cerebrovascular disorders, previous stroke, hydrocephalus, and intra-cranial mass documented by MRI; b) a history of traumatic brain injury or another neurological disease (e.g. seizures, choreoathetosis, cerebellar ataxia); c) another extrapyramidal syndrome (e.g. Parkinson disease, Lewy Body Disease, Vascular Parkinsonism, Multiple Systemic Atrophy) according to current clinical criteria; d) significant medical problems (e.g. poorly controlled diabetes or hypertension; cancer within the past 5 years; clinically significant hepatic, renal, cardiac, or pulmonary disorders); e) major depressive disorder, bipolar disorder, schizophrenia, substance abuse disorder, or mental retardation according to criteria of the DSM-IV.
99mTc-ECD SPECT acquisition
For brain functional data comparisons, a group of healthy subjects (n = 15, mean age ± SD = 56.3 ± 15.4) were recruited among patients' spouses or relatives and were included as normal controls. They were interviewed, assessed for neurological or cognitive dysfunction, evaluated for diseases that were exclusion criteria for the patients group, and underwent a brain SPECT study.
Both patients and controls were administered an intravenous injection of 1110 MBq 99mTc-ECD (ethylcysteinate dimer, Neurolite, Bristol-Myers Squibb Pharma) in a rest condition, lying supine in a quiet, dimlylit room.
All subjects were imaged using a dual-head rotating gamma camera (VG MILLENIUM GE) fitted with a low energy, high-resolution collimator, 30 minutes after intravenous injection of 99mTc-ECD.
A 128 × 128 pixel matrix was used for image acquisition with 120 views over a 360° orbit (in 3° step) with a pixel size and slice thickness of 1 mm, in 27 min or more if total counts were lower than 5 × 106. Image reconstruction was performed by a ramp filtered-back projection and three-dimensionally smoothed with a Metz filter (order 3, enhancement 1.24, FWHM 6.7 mm, cut-off 0.61 cycles cm-1). The reconstructed images were corrected for gamma ray attenuation using the Chang method (attenuation coefficient: 0.11 cm-1).
Image pre-processing and analysis
Statistical Parametric Mapping (SPM2, Welcome Department of Cognitive Neurology, University College, London), and Matlab 6.1 (Mathworks Inc., Sherborn, MA) were used for image pre-processing. Images were spatially normalized to a reference stereotactic template (Montreal Neurological Institute, MNI), and smoothed by a Gaussian kernel of 8 × 8 × 8 mm FWHM SPECT data analysis was performed in blind to clinical and genetic diagnosis.
Global differences in the distribution, and the effect of age on the tracer's uptake were covariated out for all voxels. Comparisons across the different groups were made using t-statistics with appropriate linear contrasts . We considered any cluster above a statistical threshold set at P < 0.001.
We performed the following group comparisons: (1) each FTLD group, i.e. bvFTD, SD, PNFA, PSP and CBD vs. controls, in order to explore the hypoperfusion patterns according to clinical diagnosis, and (2) FTLD patients belonging to each Latent Class group vs. controls, to explore the hypoperfusion patterns according to latent classes.
Latent Profile Analysis (LPA) was used to determine the number and composition of groups in which participants aggregated on the basis of their indicator values.
LPA, as Latent Class Analysis (LCA), is a latent variable model that serves to cluster subjects. LCA explains the clustering of cases, based on the relationship of a set of observed (manifest) categorical indicators by assuming that the patterns of values are determined by a latent (unobserved) categorical variable. Inversely, LPA allows continuous indicators to be present. For both, the number of categories of the latent variables (i.e. number of latent classes) represents the number of different clusters in subjects. In the classic form of the LPA used here, observed continuous indicators within each latent class were assumed to be uncorrelated. In other words, the model supposes that the correlation matrix between continuous outcomes is due to unobserved heterogeneity, i.e. the pairwise relationships between the continuous outcomes are due to mixing an unknown number of different classes of individuals, each having unrelated outcomes. This is also referred as conditional independence assumption, with the idea that if a sufficient number of classes is introduced, the conditional independence is more likely to hold. Thus, the latent categorical variable captures population heterogeneity, and the parameters of the model are: latent class prevalences, indicator means within latent classes, and indicator variances common to all latent classes.
We performed a sequence of five LPA models, from one to five classes, with varying across-class indicator means, and across-class common indicator variances. Neuropsychological performances (MMSE, Raven Colored Progressive Matrices, Controlled Oral Word Association Test, Category Fluency, Clock Drawing Test, Rey Complex Figure Copy and Recall, Story Recall Test, Digit Span, Trail Making Test A and B, Token Test, and De Renzi Imitation Test), functional impairment (IADL, BADL), and behavioural disturbance (NPI, FBI) and motor impairment (UPDRS-III) were introduced in the LPA as observed continuous indicators. UPDRS-III and De Renzi Imitation Test were excluded because they resulted uncorrelated with the previous variables in a preliminary descriptive data analysis.
Model parameters were estimated using Maximum Likelihood Estimator (MLE) based on the Expectation Maximization (EM) algorithm. The Akaike's Information Criterion (AIC = -2 × model log-likelihood + 2 × number of model parameters) and Bayesian Information Criterion (BIC = -2 × model log-likelihood + log(n) × number of model parameters) were computed to compare such competing models, and the selected model was the one minimizing either AIC or BIC .
Using the entropy index, the quality of the resulting classification was also evaluated in terms of the separation of the latent classes. Entropy denotes how possible it is to predict class membership given the observed indicators. Values range from 0 to 1, and high values (>0.90) indicate that the latent classes are highly discriminative.
The statistical significance of the parameter estimates were evaluated by t-tests (= parameter/standard error) considering robust standard errors. Multiple comparisons procedures based on the Fisher's Least Significant Difference (LSD) testing of across-class mean estimates were used to select discriminative indicators. The significance level was established at P < 0.05, two-sided.
All models were fit using Mplus software (version 3.13 for Windows), and full details of the computing approach can be found in Muthèn & Muthèn user's guide .
Ninety-seven patients entered the study. Demographic and clinical characteristics according to clinical diagnosis are reported in Table 1. Overall, FTLD patients were mild for global cognitive decline. BvFTD diagnosis was the most prevalent (42.2%), SD and PNFA diagnoses were less prevalent (5–6%). BvFTD showed higher behavioural disturbances (NPI and FBI-AB) compared to SD and PNFA patients, who had a different pattern of behavioural disturbances (as illustrated by high loadings in FBI-A but low scores in FBI-B); whilst CBD and PSP, as expected, showed the worst motor impairment.
Neuropsychological profile in bvFTD, SD, PNFA, CBD, and PSP patients, and the number of patients with pathological scores in each group, is reported in Table 2. Neuropsychological tests which resulted pathological in more than 50% of cases, according to Italian normative data, are highlighted: bvFTD showed greater behavioural disturbances, and SD were more impaired in verbal and non-verbal memory (Short Story and recall of Rey Complex Figure), category fluency, and executive functions (Trail Making B); PNFA patients, as expected, had language deficits (verbal and category fluency) and were more impaired in visuo-spatial skills (Trail Making A); while CBD and PSP showed deficits in visuo-spatial skills (copy of Rey Complex Figure and Trail Making A). Pathological scores of the De Renzi Imitation test were found in 43.8% of CBD patients because of the mild stage of the disease.
Functional correlates of clinical diagnosis
SPM2 was applied to SPECT scans, which were subgrouped according to clinical diagnosis (total number = 50; bvFTD = 20, SD = 3, PNFA = 2, CBD = 11, and PSP = 14). As reported in Figure 1, bvFTD showed hypoperfusion in medial frontal cortex (x, y, z = 6, 16, -12; T = 4.35, cluster size = 845; and -4,32,12; T = 3.97, cluster size = 834) and left temporal cortex (-16,12,-36; T = 3.92, cluster size = 347); SD patients had significant hypoperfusion in left temporal pole (-44,-4,-32; T = 4.87, cluster size = 1162); PNFA showed an involvement of both left frontal cortex (-32,54,20; T = 4.42, cluster size = 230) and left inferior temporal gyrus (-58,-32,-20; T = 4.09, cluster size = 167); PSP patients had significant involvement in medial frontal cortex (-2,30,16; T = 4.23, cluster size = 3846), dorsolateral frontal cortex (-48,18,24; T = 4.41, cluster size = 226) and basal ganglia and mesencephalon; CBD patients showed hypoperfusion in left inferior parietal lobe (-46,-52,28; T = 3.49, cluster size = 160), right superior temporal gyrus (36,-30,8; T = 3.77, cluster size = 141), and left uncus and parahippocampal region (-18,2,-32, T = 4.02; cluster size = 312).
Latent Profile Analysis
LPA models were fitted with 16 variables, that had from 1 to 5 LCs. Among 97 FTLD patients, 5 were excluded because of missing values. The fit statistics suggested that the model with 4 LCs had the best fit, producing minimum AIC and BIC values, and having the highest separation of the LCs evaluated by entropy measure (see Table 3). The second best model was the LPA model with 3 LCs. This model was preferred because the estimated mean profiles were more interpretable than those with 4 LCs. Additionally, the reduction in BIC statistic from 3 to 4 classes was close to zero [BIC(4) = 5401 vs. BIC(3) = 5407], and the entropy measures were close to 1 and comparable [entropy (4) = 0.963 vs. entropy (3) = 0.951].
Table 4 shows the parameter estimates for the 3 selected LCs. These parameters represent the latent classes' prevalences, the latent class specific mean profiles and the common variances of 16 variables considered in the LPA model.
Of the total sample, 18 (18.5%) patients belonged to Latent Class 1 (LC1), 41 (42.3%) to Latent Class 2 (LC2), and 38 (39.2%) to Latent Class 3 (LC3).
As shown in Figure 2, these three LCs were significantly separated from each other.
Using statistically significant pairwise mean differences provided by multiple comparisons procedure which was based on the Fisher's Least Significant Difference (LSD) testing, the latent classes are described as follows: LC1 denoted subjects with high means on behavioural disturbances (FBI-A, FBI-B, and NPI) and daily living functional impairment (BADL, IADL) compared to the other two classes. LC3 was defined by subjects with fewer cognitive disturbances measured by this neuropsychological assessment compared to those in LC1 and LC2. Finally, LC2 represented the patients with fewer behavioural disturbances but an equal cognitive profile compared to LC1 patients. In addition, LC2 shared identical behavioural deficits, but have more pronounced cognitive impairment than LC3 patients.
In clinical terms, behavioural disturbances, such as disinhibition and abnormal social conduct (as demonstrated by high FBI-B scores), characterized LC1 patients. LC2 patients showed a profile of substantial cognitive impairment with in particular a poor performance in the Trail Making B test. LC3 patients showed less cognitive impairment than the other two classes, and behavioural disturbances similar to LC2 patients. In order to better define the behavioural aspect of LC3 patients, we therefore considered the subscores of the behavioural profile. We found that LC3 and LC2 mainly differed for a greater prevalence of depressive symptoms in the former (data not shown).
Thus, we adopted the following clinical labels for the 3 LCs: "pseudomanic behaviour" endophenotype for LC1 and "pseudodepressed behaviour" endophenotype for LC3 (see reference 34); LC2 was named as "cognitive" endophenotype.
Functional correlates of Latent Classes
Functional SPECT data were then analysed according to the LCs. Fifty patients out of 97 underwent a SPECT scan. Nine patients belonging to LC1, 17 belonging to LC2, and 24 belonging to LC3 were compared to control subjects, respectively.
As shown in Figure 3, distinct functional patterns were found in LC1, LC2, and LC3 subgroups.
Comparisons of LC1 ("pseudomanic behaviour" endophenotype) vs. controls showed significant hypoperfusion in medial frontal cortex (x, y, z = -8, 50, 16; T = 5.19, cluster size = 1167), in the orbitobasal frontal cortex (0,12 -16; T = 4.52, cluster size = 871), and in left temporal pole (-38,14,-24; T = 3.82, cluster size = 171).
LC2 ("cognitive" endophenotype) vs. controls showed comparable hypoperfusion in the left temporal pole (-14,-4,-24; T = 4.76, cluster size = 457) and in subcortical brain region (-2,-6,-12; T = 4.76, cluster size = 457).
LC3 ("pseudodepressed behaviour" endophenotype) showed significant hypoperfusion in left temporal pole (-18,8,-36; T = 4.31, cluster size = 807), right dorsolateral frontal cortex (10,52,8; T = 4.86, cluster size = 506), and insula (-54,10,16; T = 4.18, cluster size = 256; 36,-30,8; T = 4.45, cluster size = 275; 42,4,0; T = 3.56, cluster size = 51).
Clinical diagnosis and Latent Class comparisons
Finally, LCs were compared to the clinical diagnosis.
In Table 5 and Figure 4, the analysis of proportion of clinical diagnoses within LCs was reported. bvFTD patients mainly belong to LC1 (the pseudomanic behaviour endophenotype), but there was a high overlap of different clinical diagnoses in LC2 and LC3.
The aim of this study was to establish a data-driven nosology and to provide the neuroimaging correlates in FTLD. This analysis suggested a division into three groups, clearly distinguished by specific functional hypoperfusion patterns.
The clinical, pathological and genetic heterogeneity of FTLD accounts for its present nosological controversy, and opens the question of whether this generic label represents a unique syndrome best viewed as complex, or if it includes different and distinct entities.
At present, FTLD classification relies upon clinical symptoms at disease presentation, but the convergence of the different clinical pictures in overlapping syndromes and their poor concordance with pathology, made of the FTLD clinical diagnosis a challenging problem . We currently differentiate FTLD in bvFTD, SD, PNFA, PSP, CBD, and these clinical distinctions may also have functional correspondences (see Figure 1). The mismatch, however, between clinical pictures and pathology makes the present classification largely unsatisfactory. This suggests the need of an alternative explanation to describe FTDL discrete clinical presentations.
In this study, we tried to disentangle these issues, employing the open statistical approach provided by Latent Profile Analysis and applying it to a large sample of FTLD patients. Our aim was to single out which, and how many clinical presentations could be generated by a statistical analysis blinded to an extended cognitive/behavioural data set in FTLD patients.
Latent Profile Analysis posits that a heterogeneous group can be reduced to several homogeneous subgroups through evaluating and then minimizing the pairwise correlations among responses across multiple continuous variables. Thus, Latent Profile Analysis is capable of determining the number and composition of unobserved latent classes that produce observed data. This approach is particularly useful when there is evidence that certain symptoms co-aggregate at above normal levels (that is, symptoms that are beyond what is usually seen in patients who present certain syndrome patterns) to form distinct clusters.
In the present study, cognitive performance, the severity of behavioural symptoms, and functional impairment were examined in a large sample of FTLD patients, and three distinct clusters were detected, named "pseudomanic behaviour", "cognitive" and "pseudodepressed behaviour".
The first, i.e. LC1, was represented by greater behavioural disturbances, such as dishinibition and abnormal social conduct, as evidenced in FBI and NPI scores. The concomitant impairment in BADL and IADL in these patients is therefore the result of inadequacy or disinhibition rather than the consequence of the cognitive decline. The second LC2 profile was underscored by a "cognitive" endophenotype, mainly characterized by executive dysfunction. The third LC3 showed better performances in neuropsychological test scores compared to the other two LCs, and subtle behavioural abnormalities, mainly represented by depressive symptoms.
It is noteworthy to mention that motor impairment, measured by UPDRS-III scale, was not used to define the latent classes, and was uncorrelated with either cognitive or behavioural profiles, suggesting an independence between motor aspects and cognitive/behavioural performances.
Thus, referring to the nosology of FTLD, our data-driven approach identified three clinical syndromes. In the same vein, a recent experimental work applied a similar approach, i.e. Factor Analysis, to clarify the classification issue in Progressive Aphasia .
As a second goal of the present study, the SPECT imaging modality and Statistical Parametric Mapping (SPM2) were used to identify functional correspondence of the FTLD as generated by Latent Profile Analysis. The SPM2 method was applied to verify if the generated LCs depended on different stage of the disease, or if they were the expression of three different endophenotypes.
The three clusters obtained by Latent Profile Analysis were underscored by specific functional correlates. Hypoperfusion in frontal medial and orbitobasal cortex was found in the "pseudomanic behaviour" endophenotype, subcortical brain region hypoperfusion was detected in the "cognitive" endophenotype, hypoperfusion in the dorsolateral frontal cortex and insula characterised the "pseudodepressed behaviour" endophenotype (Figure 3). These data provide evidence that specific neurofunctional-symptom cluster relationship can be delineated in patients with FTLD, thus excluding that these latent classes represent different disease stages.
Interestingly, the pseudomanic and pseudodepressed endophenotypes paralleled previous behavioural and metabolic findings reported in a group of FTLD patients . The main subset of symptoms in "cognitive" endophenotye, such as executive dysfunctions, can well be attributed to impairment of the dorsolateral-frontal circuit at the basal ganglia level; in fact, when data were explored at a lower threshold (P < 0.005), not only subcortical brain regions, but also dorsolateral frontal cortex was involved (-32,58,16; T = 2.87, cluster size = 101).
An increasing number of studies evaluating brain tissue volume and metabolic function in dementia demonstrate that regional tissue loss, or hypometabolism, correlates with specific cognitive or behavioural impairment in FTLD [35, 4]. FTLD patients, however, had profuse cognitive and behavioural abnormalities, thus suggesting that all these symptoms could have had a common neurofunctional basis . To date, few studies in either dementia or focal lesions has systematically examined the functional correlates of clustered behavioural and neuropsychological symptoms, such as here conducted by Latent Profile Analysis, instead of individual symptoms. We also performed the SPM2 analysis on the basis of pre-defined criteria (see Figure 1), confirming previous neuroimaging findings [7, 10, 11].
The present results suggest that FTLD syndromes lie on a continuum rather then existing as independence entities. Latent Profile Analysis approach demonstrated that FTLD can be summarised into three different clinical categories with a poor concordance with the usual clinical classification. Indeed, despite of a higher level of concordance in diagnosis distribution between LC1 and bvFTD, the other two LCs, i.e. LC2 and LC3, are otherwise variably represented (see Figure 4).
It has been recently reported that there is a convergence of the syndromes in the course of the disease . In the future, it would be interesting to evaluate the clinical and overlapping aspects of these clusters over time.
Notwithstanding, approaches based on standardised assessment, similar or different to those presented in this study, are crucial for a clear-cut description of the wide range of features, both behavioural and cognitive, that may characterise FTLD and related disorders.
The results of this study should be replicated in other sample size cross-sectional studies, and these should be performed to determine if the same endophenotypes remain stable across studies. We indeed acknowledge the need of prognostic outcomes to prove the usefulness of the present scheme. Moreover, neuropathological confirmation would be necessary to further understand the relationship between clinical and neuropathological endophenotypes. In an era of treatment that targets disease-mechanism, it would be desirable that Latent Profile Analysis will be used to further discriminate molecular/genetic determinants of FTLD pathology.
In conclusions, among the mild FTLD variants, characterised by a wide clinical overlap, specific clinical and functional neural networks may be identified at the beginning of the disease. Further longitudinal studies will be devoted to analysing the evolution of such clusters in order to delineate the pattern of disease progression.
Frontotemporal Lobar Degeneration
Single Photon Emission Tomography
behavioural variant of frontotemporal dementia
Progressive Non-fluent Aphasia
Progressive Supranuclear Palsy
Latent Profile Analysis.
Kertesz A, Hillis A, Munoz D: Frontotemporal degeneration, Pick's Disease, Pick Complex and Ravel. Ann Neurol. 2003, 54 (S5): 1-2. 10.1002/ana.10595.
Knibb JA, Xuareb JH, Patterson K, Hodges JR: Clinical and pathological characterisation of progressive aphasia. Ann Neurol. 2006, 59: 156-65. 10.1002/ana.20700.
Kertesz A, Hudson L, Mackenzie IR, Munoz DG: The pathology and nosology of primary progressive aphasia. Neurology. 1994, 44: 2065-72.
Rosen HJ, Allison SC, Schauer GF, Gorno-Tempini ML, Weiner MW, Miller BL: Neuroanatomical correlates of behavioural disorders in dementia. Brain. 2005, 128: 2612-25. 10.1093/brain/awh628.
Hodges JR, Davies RR, Xuereb JH, Casey B, Broe M, Bak TH, Kril JJ, Halliday GM: Clinicopathological correlates in frontotemporal dementia. Ann Neurol. 2004, 56: 399-406. 10.1002/ana.20203.
Padovani A, Borroni B, Brambati SM, Agosti C, Broli M, Alonso R, Scifo P, Bellelli G, Alberici A, Gasparotti R, Perani D: Diffusion tensor imaging and voxel based morphometry study in early progressive supranuclear palsy. J Neurol Neurosurg Psychiatry. 2006, 77: 457-63. 10.1136/jnnp.2005.075713.
Miller BL, Seeley WW, Mychack P, Rosen HJ, Mena I, Boone K: Neuroanatomy of the self: evidence from patients with frontotemporal dementia. Neurology. 2001, 11: 817-21.
Shi J, Shaw CL, Du Plessis D, Richardson AM, Bailey KL, Julien C, Stopford C, Thompson J, Varma A, Craufurd D, Tian J, Pickering-Brown S, Neary D, Snowden JS, Mann DM: Histopathological changes underlying frontotemporal lobar degeneration with clinicopathological correlation. Acta Neuropathol. 2005, 110: 501-12. 10.1007/s00401-005-1079-4.
Kertesz A, McMonagle P, Blair M, Davidson W, Munoz DG: The evolution and pathology of frontotemporal dementia. Brain. 2005, 128: 1996-2005. 10.1093/brain/awh598.
Gorno-Tempini ML, Dronkers NF, Rankin KP, Ogar JM, Phengrasamy L, Rosen HJ, Johnson JK, Weiner MW, Miller BL: Cognition and anatomy in three variants of primary progressive aphasia. Ann Neurol. 2004, 55: 335-46. 10.1002/ana.10825.
Chapman SB, Bonte FJ, Wong SB, Zientz JN, Hynan LS, Harris TS, Gorman AR, Roney CA, Lipton AM: Convergence of connected language and SPECT in variants of frontotemporal lobar degeneration. Alzheimer Dis Assoc Disord. 2005, 19: 202-13. 10.1097/01.wad.0000189050.41064.03.
Liu W, Miller BL, Kramer JH, Rankin K, Wyss-Coray C, Gearhart R, Phengrasamy L, Weiner M, Rosen HJ: Behavioral disorders in the frontal and temporal variants of frontotemporal dementia. Neurology. 2004, 62: 742-48.
Bartholomew DJ, Knott M: Latent Variable Models and Factor Analysis. 1999, London: Arnold
Neary D, Snowden J, Gustafson L, Passant U, Stuss D, Black S, Freedman M, Kertesz A, Robert P, Albert M, Boone K, Miller B, Cummings J, Benson D: Frontotemporal Lobar degeneration: a consensus on clinical diagnostic criteria. Neurology. 1998, 51: 1546-54.
McKhann GM, Albert MS, Grossman M, Miller B, Dickson D, Trojanowski JQ, Work Group on Frontotemporal Dementia and Pick's Disease: Clinical and pathological diagnosis of frontotemporal dementia: report of the Work Group on Frontotemporal Dementia and Pick's Disease. Arch Neurol. 2001, 58: 1803-09. 10.1001/archneur.58.11.1803.
Louis ED, Lynch T, Marder K, Fahn S: Reliability of patient completion of the historical section of the United Parkinson's Disease Rating Scale. Mov Disord. 1996, 11: 185-92. 10.1002/mds.870110212.
Folstein MF, Folstein SE, McHugh PR: "Mini-Mental State": a practical method for grading the cognitive state of patients for the clinician. J Psychiatr Res. 1975, 12: 189-98. 10.1016/0022-3956(75)90026-6.
Bingham WC, Burke HR, Murray S: Raven's Progressive Matrices: construct validity. J Psycgol. 1966, 62: 205-9.
Isaacs B, Kennie AT: The Set test as an aid to the detection of dementia in old people. Br J Psychiatry. 1973, 123: 467-70.
Wolf-Klein GP, Silverstone FA, Levy AP, Brod MS: Screening for Alzheimer's disease by clock drawing. J Am Geriatr Soc. 1989, 37: 730-34.
Loring DW, Martin RC, Meador KJ: Psychometric construnction of the Rey-Osterrieth Complex Figure. Arch Clin Neuropsych. 1990, 5: 1-14. 10.1016/0887-6177(90)90002-7.
Babcock H, Levy L: The measurement of mental efficiency of mental functioning (revised examination). Test and manual of directions. 1940, Chicago: CH Stoelting
Blackburn HL, Benton AL: Revised administration and scoring of the digit span test. J Consult Psychol. 1957, 21: 139-43. 10.1037/h0047235.
Reitan RM: Validity of the Trail Making Test as an indicator of organic brain damage. Perceptual and motor skills. 1958, 8: 271-76.
De Renzi E, Vignolo LA: The Token Test: a sensitive test to detect receptive disturbances in aphasics. Brain. 1962, 85: 665-78. 10.1093/brain/85.4.665.
De Renzi E, Motti F, Nichelli P: Imitating gestures. A quantitative approach to ideomotor apraxia. Arch Neurol. 1980, 37: 6-10.
Lawton MP, Broody EM: Assessment of older people: self-maintaining and instrumental activities of daily living. The Gerontologist. 1969, 9: 179-86.
Sheikh K, Smith DS, Meade TW, Goldenberg E, Brennan PJ, Kinsella G: Repeatibility and validity of a modified Activities of Daily Living (ADL) Index in studies of chronic disability. Int Rehabil Med. 1979, 1: 51-58.
Cummings JL, Mega M, Gray K, Rosenberg-Thompson S, Carusi DA, Gornbein J: The Neuropsychiatric Inventory: comprehensive assessment of psychopathology in dementia. Neurology. 1994, 44: 2308-14.
Kertesz A, Nadkarni N, Davidson W, Thomas AW: The Frontal Behavioral Inventory in the differential diagnosis of frontotemporal dementia. J Int Neuropsychol Soc. 2000, 6: 460-68. 10.1017/S1355617700644041.
Friston KJ, Holmes AP, Worsley KJ, Poline J-B, Frith CD, Frackowiack RSJ: Statistical parametric maps in functional imaging: a general linear approach. Hum Brain Mapp. 1995, 2: 189-210. 10.1002/hbm.460020402.
Burnham KP, Anderson DR: Model Selection and Inference. A Practical Information Theoretic Approach. 2002, Springer-Verlag, New York, 2
Muthén LK, Muthén BO: How to use a Monte Carlo study to decide on sample size and determine power. Structural Equation Modeling. 2002, 9: 599-620. 10.1207/S15328007SEM0904_8.
Franceschi M, Anchisi D, Pelati O, Zuffi M, Matarrese M, Moresco RM, Fazio F, Perani D: Glucose metabolism and serotonin receptors in the frontotemporal lobe degeneration. Ann Neurol. 2005, 57: 216-25. 10.1002/ana.20365.
Nadeau SE: Alzheimer's disease as a window to neural mechanisms of cognition. Neurology. 2003, 61: 1470-71.
Salmon E, Kerrouche N, Herholz K, Perani D, Holthoff V, Beuthien-Baumann B, Degueldre C, Lemaire C, Luxen A, Baron JC, Collette F, Garraux G: Decomposition of metabolic brain clusters in the frontal variant of frontotemporal dementia. Neuroimage. 2006, 30: 871-78. 10.1016/j.neuroimage.2005.10.016.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2377/7/9/prepub
The authors wish to thank the patients and their families for taking part into this study. The authors are indebted to Andrea Rudiak for editing the manuscript.
The author(s) declare that they have no competing interests.
BB conceived of the study, and participated in its design and coordination and in the draft of the manuscript; MG participated in the design of the study, performed statistical analysis and revised the manuscript critically for intellectual content; DP participated in the design of the study, in neuroimaging analyses and revised the manuscript critically for intellectual content; CA and AA performed the clinical evaluation of the patients and have made substantial contribution to interpretation of data; BP has made substantial contributions in the acquisition of the data; MDL has been involved in the study design and in revising it critically for important intellectual content; AP conceived of the study, and participated in its design and in coordination and drafted the manuscript. All authors read and approved the final manuscript.