- Case report
- Open Access
An autopsied case report of spastic paraplegia with thin corpus callosum carrying a novel mutation in the SPG11 gene: widespread degeneration with eosinophilic inclusions
BMC Neurology volume 22, Article number: 2 (2022)
The detailed neuropathological features of patients with autosomal recessive hereditary spastic paraplegia with a thin corpus callosum (TCC) and SPG11 mutations are poorly understood, as only a few autopsies have been reported. Herein, we describe the clinicopathological findings of a patient with this disease who received long-term care at our medical facility.
A Japanese man exhibited a mild developmental delay in early childhood and intellectual disability, followed by the appearance of a spastic gait by age 13. At the age of 25 years, he became bedridden and needed a ventilator. Genetic analysis revealed a homozygous splice site variant in the SPG11 gene (c. 4162–2A > G) after the provision of genetic counselling and acquisition of informed consent from his parents. He died of pneumonia at the age of 44. His brain weighed 967 g and was characterized by a TCC, and his spinal cord was flattened. Microscopically, degeneration was observed in the posterior spinocerebellar tract, the gracile fasciculus, and the posterior column in addition to the corticospinal tract. Marked neuronal loss and gliosis were observed in the anterior horn, Clarke’s column, and hypoglossal and facial nuclei. Various types of neurons, in addition to motor neurons, showed coarse eosinophilic granules that were immunoreactive for p62. The loss of pigmented neurons with gliosis was apparent in both the substantia nigra and locus coeruleus. Lateral geniculate body degeneration was a characteristic feature of this patient. Furthermore, peripheral Lewy body-related α-synucleinopathy and scattered α-synuclein–immunoreactive neurites in the locus coeruleus and reticular formation of the brainstem were observed.
In patients with hereditary spastic paraplegia with SPG11 mutations, a variety of clinical phenotypes develop due to widespread lesions containing p62-immunoreactive neuronal cytoplasmic inclusions. We herein report the lateral geniculate body as another degenerative site related to SPG11-related pathologies that should be studied in future investigations.
Autosomal recessive hereditary spastic paraplegia with a thin corpus callosum (ARHSP-TCC) is characterized by slowly progressive spastic paraparesis and intellectual disability and is reported to be a clinicopathologically unique complicated form of HSP in studies on Japanese patients [1, 2]. The genetic causes of ARHSP-TCC have been incrementally elucidated. In 1999, Martinez et al. performed genetic linkage analysis, and the results suggested a new locus for ARHSP linked to chromosome 15q13–15 in families with ARHSP-TCC from America and Europe; this locus was termed SPG11 because it was the eleventh gene discovered to underlie HSP . Later, Shibasaki et al. reported 10 Japanese ARHSP-TCC families, including two previously reported families [2, 4], who also showed linkage to the 15q13–15 locus . After that, Stevanin et al. analysed HSP with SPG11 mutations in index patients and identified mutations in the KIAA1840 gene, which encodes spatacsin . From these findings, SPG11 mutations are now widely known as the most common cause of ARHSP-TCC [6,7,8,9,10,11,12]. However, the detailed neuropathological features of ARHSP-TCC with SPG11 mutations are poorly understood due to the limited number of autopsy reports [13,14,15].
Here, we describe the clinical and pathological characteristics of an autopsied patient with spastic paraplegia with a TCC and a homozygous splice site variant in the SPG11 gene (NM_025137.3: c.4162–2A > G).
The patient was a Japanese man, and his parents were consanguineous (Fig. 1A). He was born full-term via vaginal delivery. He exhibited a mild developmental delay in early childhood and was diagnosed with an intellectual disability at 8 years old. Gait disturbance with an equinovarus foot deformity and leg spasticity was observed at the age of 13 years. At the age of 19, he was admitted to our hospital. At that time, his IQ was 35 based on the Tanaka-Binet Intelligence Scale. His eye movements were saccadic without nystagmus, and he had mild dysarthria. He showed muscle weakness in both lower limbs and a spastic gait. He did not have bradykinesia, muscle rigidity, cerebellar ataxia, or sensory disturbances. Magnetic resonance imaging of the brain showed global thinning of the corpus callosum, a wide cavum verge and cavum septi pellucidi, an abnormally high area of intensity in the periventricular white matter, and hypoplasia or atrophy of the frontal lobe . Based on these findings, he was diagnosed with HSP with a TCC.
At the age of 20 years, he developed clumsiness and weakness in the upper limbs. The following year, he was not able to walk by himself. At the age of 23 years, nerve conduction studies showed mild delays in the conduction velocities in the motor nerves of the upper and lower limbs and in the sensory nerves of the upper limbs. Atrophy of his frontal and temporal lobes was revealed at the age of 24 years by brain computed tomography (Fig. 2). At the age of 25 years, he became bedridden, dysarthria progressed, and dysphagia and a depressive state appeared. Therefore, he required feeding by a nasal tube. One month later, he underwent sudden respiratory arrest and was thus intubated and required a ventilator. Respiratory arrest occurred while his arterial oxygen saturation of pulse oxymetry and heart rate were monitored; therefore, he did not have cardiac arrest, and his level of consciousness did not change before and after the event. One month later, his spontaneous breathing recovered, but due to unstable respiratory functioning, he underwent a tracheostomy. At the age of 39 years, he became quadriplegic, with absent deep tendon reflexes and distal dominant muscle amyotrophy, and he required permanent ventilation from this point forward. Ocular fixation was observed, but his pupil light reflex was delayed. He died of pneumonia at the age of 44 years, 31 years after the onset of spasticity. He did not have seizures at any time during the course of his disease.
Genetic analysis results
A detailed description of genetic analysis methods is provided in Additional file 1. TruSight One sequencing panels revealed a mutation around exon 25 of SPG11. Direct Sanger sequencing of SPG11 revealed a homozygous variant of SPG11 (c. 4162 − 2A > G) (Fig. 1B). A heterozygous variant at the same location was identified in both parents. This variant was located at the splice acceptor site of intron 24 of SPG11 and was predicted to be a disease-causing (very strong pathogenicity, PVS1) mutation of a known causative gene of ARHSP-TCC according to the American College of Medical Genetics and Genomics standards and guidelines . Furthermore, we could not find this variant in ClinVar (https://www.ncbi.nlm.nih.gov/clinvar/), the Japanese genome database of Human Genetic Variation (http://www.hgvd.genome.med.kyoto-u.ac.jp), or the Japanese Reference Genome version 1 (https://jmorp.megabank.tohoku.ac.jp/202001/variants).
An autopsy was performed 13.5 h after death. A detailed description of neuropathological examination methods is provided in Additional file 1. The patient had a right lung abscess and left focal aspiration pneumonia, and his cause of death was respiratory failure.
In the brain, which weighed 967 g, the cerebrum, brainstem, and cerebellum were macroscopically small (Fig. 3A, B), and atrophy was particularly noticeable in the frontal lobe and the precentral gyrus (Fig. 3A, B). The corpus callosum was extremely thinned over the entire rostrocaudal length (Fig. 3B, C). The volumes of the cerebral cortex and white matter were decreased (Fig. 3C). The caudate nucleus and thalamus exhibited mild atrophy, and the thalamus was brownish in colour (Fig. 3C). In the brainstem, both the substantia nigra and locus coeruleus showed depigmentation (Fig. 3D, E). Atrophy of the optic nerve was not observed (Fig. 3A). The whole spinal cord was flattened, especially in the thoracic cord. Atrophy of the ventral predominant spinal roots was observed. Microscopically, in the spinal cord, the corticospinal tract, the posterior spinocerebellar tract, the posterior column (including gracile fasciculus and the intermediate root zone), and the anterolateral funiculus showed bilateral decreases in myelin and axonal staining (Fig. 4A). Marked neuronal loss and gliosis were observed in the anterior horn at all levels of the spinal cord (Fig. 4B, C). Moreover, Onuf’s nuclei and Clarke’s column also showed severe neuronal loss and gliosis (Fig. 4D, E), while the intermediolateral nuclei in the thoracic and sacral spinal cord regions were relatively spared. Severe neuronal loss and gliosis were also observed in the hypoglossal and facial nuclei, while the abducens and oculomotor nuclei were relatively preserved. Coarse eosinophilic granules were observed in the remaining neurons of all of these motor nuclei (Fig. 4F). These eosinophilic inclusions were immunoreactive for p62 and ubiquitin (Fig. 4G) but not for phosphorylated TAR DNA-binding protein 43 kDa or cystatin C. The p62-immunoreactive neuronal cytoplasmic inclusions (NCIs) were more numerous and widespread than the NCIs identified by haematoxylin-eosin staining. Table 1 shows the findings of neuronal loss, gliosis, and the frequency of p62-immunoreactive NCIs in various regions.
The medullary pyramid was atrophic and showed myelin and axonal loss, whereas the cerebral peduncle showed only a dimple in the middle. The loss of pigmented neurons with gliosis was apparent in both the substantia nigra and locus coeruleus. In the cerebellum, depletion of Purkinje cells with proliferation of Bergmann glia and isomorphic gliosis in the cerebellar white matter were predominantly observed in the vermis, and grumose degeneration—in addition to moderate neuronal loss and gliosis—was observed in the cerebellar dentate nucleus. The thalamus showed severe neuronal loss and gliosis. Moderate neuronal loss and gliosis were also observed in the striatum. The amygdala exhibited prominent gliosis; on the other hand, the hippocampus showed mild neuronal loss and gliosis. The rare appearance of p62-immunoreactive NCIs in the limbic areas was different from that observed in the other areas (Table 1). The corpus callosum was very thin (Fig. 4H) and accompanied by gliosis, but slight myelin and axonal staining remained (Fig. 4I, J). The neurons in the cerebral cortex appeared to be small, and the cortical widths seemed to be slightly narrow, especially in the frontal cortex; however, the layered structures were maintained. The precentral gyrus showed moderate neuronal loss and gliosis and a decreased number of radiating fibres. Betz cells became so atrophic that they were difficult to identify (Fig. 4K). To a lesser extent, the frontal lobe showed neuronal loss and gliosis. The volume of cerebral white matter was reduced, but some myelin staining remained. The lateral geniculate body (LGB) showed marked neuronal loss and gliosis in all layers (Fig. 4L, M), with secondary degeneration being observed in the optic radiation and the striate cortex. The optic nerve was well preserved. The sural nerve exhibited a severely decreased number of myelinated fibres, and many Nageotte nodules were observed in the spinal dorsal root ganglia.
As a comorbid pathology, Lewy body-related α-synucleinopathy (LBAS) was abundant in the peripheral nervous systems, including sympathetic ganglia, heart, skin, and gastrointestinal tract, dorsal motor nucleus of the vagus nerve, and solitary nuclei (Fig. 4N). Scattered α-synuclein–immunoreactive neurites were observed in the locus coeruleus and reticular formation of the brainstem. No LBAS was observed in the substantia nigra, basal ganglia, olfactory bulb, limbic systems, or cerebrum. Senile changes were minimal.
Discussion and conclusions
Herein, we report an autopsied Japanese patient with HSP-TCC who had a novel mutation in the SPG11 gene, which encodes spatacsin. The clinical phenotype of this patient was similar to that previously reported for HSP with mutations in SPG11 .
In the present patient and other reported autopsied patients [13, 14], common pathological features include degeneration in the upper and lower motor neuron systems as well as in the grey and white matter of the cerebral neocortex, thalamus, substantia nigra, Clarke’s nuclei, spinocerebellar tract, sural nerve, and dorsal root ganglia. Furthermore, we described an LGB lesion with p62-immunoreactive NCIs for the first time in a patient with an SPG11 mutation. Although this patient did not appear to have obvious visual abnormalities and the optic nerve was well preserved, previous reports on SPG11 patients have described decreased vision or visual evoked potential abnormalities [7, 18]. In addition, in a previous report on a Japanese patient with HSP-TCC who was not identified as having an SPG11 mutation, degeneration of the LGB was described [1, 19]. Given that SPG11 mutations are the most common type of mutation in patients with HSP-TCC in Japan, HSP with an SPG11 mutation could involve the LGB, and LGB lesions should be considered a cardinal cause of visual abnormalities in patients with SPG11 mutations. Taken together, these results suggest that SPG11 patients can present with LGB lesions, resulting in visual abnormalities.
We comprehensively evaluated the central nervous system of our patient and found p62-immunoreactive NCIs throughout, which corresponded to degenerated sites (Table 1). In contrast with the present patient, previously reported SPG11 patients showed limited distributions of p62-immunoreactive NCIs. Kuru et al. described eosinophilic granular inclusions in the substantia nigra, dentate nucleus, anterior horn, and spinal ganglia, but they did not confirm the nature of these inclusions using p62 immunostaining . Denora et al. observed p62-immunoreactive eosinophilic granular inclusions in the anterior horn of the spinal cord, hypoglossal nucleus, and dorsal root ganglia . Mori et al. observed p62-immunoreactive NCIs in the spinal cord and brainstem as well as in the subcortical nuclei and cingulate cortex; the patients reported in that study showed a somewhat broad distribution of p62-immunoreactive NCIs . The present patient had a broader distribution of p62-immunoreactive NCIs and exhibited rapid clinical progression from onset until the need for nasal tube feeding, becoming bedridden and developing respiratory failure earlier than other autopsied patients with HSP-TCC and SPG11 mutations (Table 2) [13, 14, 20]. These results raise the possibility that the widespread accumulation of p62-immunoreactive NCIs contributes to rapid clinical progression.
The patient also showed an accumulation of LBAS. The incidence of LBAS is known to increase with age , and LBAS is present in one-third of the aged population . However, our patient showed LBAS, albeit mild, despite being in his mid-forties. Genetic analysis did not reveal any already known pathological genetic variations associated with Parkinson’s disease. Kuru et al. reported that the spatacsin protein, which is encoded by SPG11, is localized in Lewy bodies (LBs), Lewy neurites, and pale bodies. Pale bodies are considered to be precursors of LBs , therefore they hypothesized that spatacsin is involved in the early stages of LB formation. The accumulation of LBAS earlier in life in our patient may provide support for their hypothesis. SPG11 mutations may accelerate the accumulation of LBAS. Future studies focusing on this hypothesis are expected.
In conclusion, we report a patient with spastic paraplegia with an SPG11 mutation who showed p62-immunoreactive NCIs throughout the central nervous system that corresponded to degenerated sites. A variety of clinical phenotypes develop due to such widespread lesions. The LGB is also a site of degeneration in patients with SPG11. Consequently, detailed neurological examinations, including visual function assessments and wide-ranging neuropathological investigations, should be conducted in patients with SPG11 in the future. Furthermore, the comorbid LBAS pathology observed in the present patient suggests the need to further evaluate the involvement of SPG11 in LB formation.
Availability of data and materials
The data that support the findings presented in this study are available from the corresponding author upon reasonable request.
Autosomal recessive hereditary spastic paraplegia
Lewy body-related α-synucleinopathy
Lateral geniculate body
Neuronal cytoplasmic inclusion
Thin corpus callosum
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The authors would like to thank the patient and patient’s family for their cooperation, Dr. Toshiki Takenouchi and Dr. Tomoko Uehara for supporting the genetic analysis, and Mr. Yutaka Koga, Ms. Mieko Harada, Ms. Kyoko Okamoto, Ms. Nobuko Naoi, and Ms. Sachiko Imai for providing technical assistance.
This work was supported by Grants-in-Aid from the Research Committee of CNS Degenerative Diseases; Research on Policy Planning and Evaluation for Rare and Intractable Diseases; Health, Labour and Welfare Sciences Research Grants; the Ministry of Health, Labour and Welfare, Japan; MEXT/JSPS KAKENHI grant number JP16H06277 (SM), and AMED grant number JP18dm0107103 (SM). The funders played no role in the design of the study, in the collection, analysis, or interpretation of data or in the writing of the manuscript.
Ethics approval and consent to participate
This study was approved by the institutional ethics committee of Tokyo Metropolitan Kita Medical and Rehabilitation Center for the Disabled (no. 2021–6). The patient’s family provided written informed consent prior to investigation.
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The patient’s parent gave written consent for their personal and clinical details along with any identifying images to be published in this study.
The authors have no competing interests to declare.
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Hayakawa, M., Matsubara, T., Mochizuki, Y. et al. An autopsied case report of spastic paraplegia with thin corpus callosum carrying a novel mutation in the SPG11 gene: widespread degeneration with eosinophilic inclusions. BMC Neurol 22, 2 (2022). https://doi.org/10.1186/s12883-021-02514-z
- Case report
- Hereditary spastic paraplegia with a thin corpus callosum
- Lateral geniculate body
- Lewy body-related α-synucleinopathy
- p62-immunoreactive neuronal cytoplasmic inclusions
- SPG11 gene